相关实验视频

Updated: Jun 3, 2025

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Published on: October 21, 2017

调节mTOR-依赖的天体细胞基质转换以缓解神经退行症

Liansheng Zhang¹, Zhengzheng Xu², Zhiheng Jia^2,3

¹Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. zhangls@ion.ac.cn.

|January 8, 2025

概括

反应性星体细胞不是不同的亚型,而是过渡状态. 神经保护性星球细胞在神经炎症中先于神经毒性状态,这是由mTOR信号调节的过程,为神经退行性疾病提供治疗点.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

在神经炎症和神经退行性疾病中,星细胞异质性至关重要.
之前的研究集中在静态的天体细胞属性,忽视动态状态.

研究的目的:

为了研究反应性星体细胞的动态状态.
阐明天体细胞转换在神经炎症和神经退行性疾病中的作用.
为了确定调节天体细胞功能的治疗点.

主要方法:

时间序列多原子测序来追踪星细胞状态.
对阿尔茨海默病 (AD) 和衰老模型中的星球细胞群的分析.
在星球细胞中mTOR信号通路的遗传和药理向.

主要成果:

星球细胞通过神经保护和神经毒性状态的过渡,而不是不同的亚型.
拉巴胺素 (mTOR) 信号通路的机械性标调节了这种过渡.
在AD和衰老中观察到这些状态的不平衡.
在小鼠模型中,用拉巴胺素或shRNA向天体细胞mTOR可降低神经毒性.

结论:

天体细胞表现出动态的功能变化,在神经炎症期间从神经保护过渡到神经毒性.
星球细胞中的mTOR信号传递是这种过渡的关键调节者.
调节天体细胞mTOR为神经退行性疾病提供了潜在的治疗策略.

更多相关视频

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

Published on: February 5, 2018

AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke

AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke

Published on: November 29, 2024

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...