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相关概念视频

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Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jun 3, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

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使用长读序列测序对表观基因组进行剖析.

Tianyuan Liu1, Ana Conesa2

  • 1Institute for Integrative Systems Biology, Spanish National Research Council, Paterna, Spain.

Nature genetics
|January 8, 2025
PubMed
概括
此摘要是机器生成的。

长读测序 (LRS) 技术能够直接检测DNA修饰,并对长DNA序列进行分析. 本综述探讨了用于表观基因组分析和理解转录动态的LRS策略.

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Targeted DNA Methylation Analysis by Next-generation Sequencing
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

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相关实验视频

Last Updated: Jun 3, 2025

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科学领域:

  • 基因组学就是基因组学.
  • 文字转录学 (Transcriptomics) 是一个学科.
  • 表观基因组学是指表观基因组学.

背景情况:

  • 来自牛津纳米孔和太平洋生物科学的单分子,长读测序 (LRS) 技术已经改变了生物研究.
  • 在单个分子层面上,LRS可直接检测DNA修饰 (例如甲基化) 和分析千基基跨越的DNA序列.

研究的目的:

  • 审查基于LRS的实验和计算策略,以表征染色体状态.
  • 突出LRS在短读测序方法上的优势.
  • 展示LRS方法的整合,用于研究染色体状态和转录动态的多奥米学研究.

主要方法:

  • 讨论LRS技术及其在表观遗传学中的应用.
  • 将LRS与短读测序方法进行比较.
  • 使用LRS的多omics数据的整合策略.

主要成果:

  • 通过整合DNA甲基化,染色质可访问性,转录因子结合和基因组修饰数据,LRS促进了全面的表观基因组分析.
  • 通过LRS,可以检测替代,新生和翻译的转录.
  • LRS提供了一种强大的工具,用于分析染色体状态及其与转录动态的关系.

结论:

  • 对于表观基因组研究和转录基因组学来说,LRS技术具有显著的优势.
  • 基于LRS的综合多组学研究对于全面了解基因调节和细胞功能至关重要.