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肺部转录基因适应中的性变态胎儿酒精谱系障碍中的性变态

Vishal D Naik¹, Dylan J Millikin¹, Daniel Moussa¹

¹Department of Obstetrics and Gynecology, C.S. Mott Center for Human Growth and Development, School of Medicine, Wayne State University, 275 E Hancock St, Rm 195, Detroit, MI, 48201, USA.

Respiratory research

|January 9, 2025

概括

发育性酒精暴露会影响胎儿肺部发育,导致基因表达和免疫反应的性二态变化. 女性胎儿肺部显示T细胞调节的显著变化,而两性都表现出肺部形态受损和降低了脉膜化.

科学领域:

生殖生物学生殖生物学
发展性毒理学发展性毒理学
免疫学免疫学免疫学

背景情况:

胎儿酒精谱系障碍 (FASD) 研究主要集中在神经效应上,忽视了肺部并发症.
呼吸道感染是新生儿发病和死亡的主要原因,对FASD对胎儿肺免疫的影响的了解有限.
现有关于FASD和肺部适应的研究表明表面活性蛋白A减少,膜巨细胞功能受损,肺炎风险增加,但缺乏对性二重形状的研究.

研究的目的:

调查发育期酒精暴露对胎儿肺形态和免疫适应的性二形效应.
为了确定特定的基因表达模式和免疫路径受孕前暴露于男性和女性胎儿肺部的酒精影响.

主要方法:

在关键的孕期期间,孕妇大鼠被暴露在酒精或双人养的对照中.
用RNA测序 (Illumina NovaSeq S4) 来分析胎儿肺组织的基因表达.
进行了组织病理学评估,以评估肺部形态和分化.

主要成果:

暴露于酒精导致胎儿体重,冠状长度和暴露幼的胎盘效率显著降低.
基因表达分析揭示了与性别相关的二分法,与男性相比,女性胎儿肺部的基因变化明显更多.
暴露于酒精的女性表现出与T细胞激活,调节和分化相关的基因下调,包括减少CD8+T细胞数量. 两性均表现出缩的肺分化,低成形,降低膜体大小和降低膜毛细体的发育.

结论:

产前酒精暴露会诱导胎儿肺部发育和免疫基因表达的性二态变化.
女性胎儿肺部受到不成比例的影响,T细胞相关基因的显著失调.
这些发现强调了在FASD研究中考虑性二态化的重要性,并突出了受影响个体对呼吸系统健康的潜在长期风险.

关键词:

酒精饮料酒精饮料酒精饮料酒精饮料胎儿胎儿胎儿的意思肺肺肺肺肺肺肺怀孕怀孕怀孕怀孕两性二形态主义是什么?

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