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喷雾诱导的基因沉默对谷物植物圈微生物群的影响

Poorva Sundararajan¹, Samrat Ghosh¹, Bekele Gelena Kelbessa¹

¹Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.

Environmental microbiome

|January 9, 2025

概括

使用双链RNA (dsRNA) 的喷雾诱导基因沉默 (SIGS) 有效地向小麦和大麦中的Fusarium头部炎症. 虽然细菌群体表现出一些变化,但核心的真菌和细菌植物界微生物群保持稳定,验证SIGS作为一种安全,环保的作物保护方法.

科学领域:

植物病理学植物病理学
微生物生态学微生物生态学
生物技术是生物技术.

背景情况:

由Fusarium graminearum引起的Fusarium头炎 (FHB) 显著降低谷物作物的产量和质量.
增加FHB发病率威胁到全球粮食安全,需要新的管理策略.
喷雾诱导基因沉默 (SIGS) 提供了一种非转基因方法,通过应用双链RNA (dsRNA) 来对抗FHB.

研究的目的:

研究dsrna喷雾对小麦和大麦植物圈中的细菌和真菌微生物群的影响.
通过检查其对非目标微生物群落的影响,评估SIGS的安全性和特异性.
为了了解菌 (Fusarium graminearum) 在植物圈微生物群中的生态相互作用.

主要方法:

使用高通量安普利康测序来分析细菌和真菌群落.
针对特定病原体基因的dsRNA治疗方法应用于小麦和大麦植物.
进行了微生物多样性,结构,组成和共发生模式的分析.

主要成果:

细菌微生物群的多样性,结构和组成被dsrna处理和宿主植物所改变.
植物圈中的真菌微生物群在dSRNA喷后基本保持不变.
无处不在的细菌和真菌属在治疗中持续存在,这表明核心微生物群的稳定性.

结论:

SIGS证明了目标特异性,对整个植物圈微生物群的影响最小.
无处不在的微生物群落的稳定性支持SIGS作为一个环保的疾病管理工具的安全性.
在谷物中,SIGS显示了对环境负责任的Fusarium头部病菌控制的巨大潜力.

关键词:

类植物 (Fusarium graminearum) 是一种植物.安普利康序列变体 (ASVs) 是一个变体.大麦大麦,大麦大麦,大麦大麦,大麦大麦.双链RNA (dsRNA) 是一种双链RNA.微生物组是一个微生物组.生物圈 (Phyllosphere) 是一个生物圈.喷雾诱导的基因沉默 (SIGS)小麦小麦小麦小麦的小麦.

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