相关实验视频
Updated: Jun 3, 2025

04:20
Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
Published on: February 9, 2024
869
FAM49B通过通过NEK9酸化稳定c-Myc来驱动结直肠癌的进展
Chen Lu1,2, Tianyu Liu2, E Yimin3
1Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
BioFactors (Oxford, England)
|January 9, 2025
概括
FAM49B基因通过激活c-Myc瘤基因来促进结直肠癌 (CRC) 的生长. 针对FAM49B或NEK9为c-Myc阳性CRC患者提供了潜在的治疗方法.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症遗传学 癌症遗传学
背景情况:
- 结肠直肠癌 (CRC) 是全球癌症死亡的主要原因.
- 在CRC病变发生过程中FAM49B的作用在很大程度上没有被描述.
- FAM49B是最近发现的,进化保存的基因,涉及各种癌症.
研究的目的:
- 为了阐明FAM49B在结直肠癌中的功能作用.
- 研究FAM49B在CRC中的作用背后的分子机制.
- 在FAM49B路径中识别潜在的治疗点.
主要方法:
- 在CRC组织和细胞系中分析FAM49B表达.
- FAM49B敲击实验,以评估对细胞增殖,迁移和入侵的影响.
- 单细胞RNA测序 (ScRNA-seq) 用于识别相互作用的基因和通路.
- 西方涂抹和无处不在测试用于研究蛋白质相互作用和修饰.
- 同免疫沉证实FAM49B和NEK9.9之间的直接相互作用.
主要成果:
- 在CRC中,FAM49B的表达上调,与预后不佳相关.
- 通过阻止细胞循环,FAM49B knockdown 抑制了CRC细胞的增殖,迁移和入侵.
- 高FAM49B表达与c-Myc瘤基因激活有关.
- 通过增强的K48无处置,FAM49B的淘汰降低了c-Myc的表达.
- NEK9被确定为FAM49B的直接相互作用伙伴,其酸化被FAM49B敲击抑制.
- 高NEK9表达也与不良的CRC预后有关.
- 针对FAM49B/NEK9/c-Myc轴抑制了FAM49B过度表达的CRC细胞中的增殖,迁移和入侵.
结论:
- FAM49B通过NEK9/c-Myc信号通路促进CRC的进展.
- FAM49B和NEK9是结直肠癌的潜在预后生物标志物.
- FAM49B/NEK9/c-Myc通路代表了对c-Myc阳性CRC的有前途的治疗标.
相关概念视频
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Induced Pluripotent Stem Cells
3.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
3.9K
Positive Regulator Molecules
5.3K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.3K
Destabilization of Microtubules
2.6K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.6K

