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来自Rhodococcus erythropolis的NAD(P) H-依赖的减少性氨酶的发现和合成应用
Ewald P J Jongkind1, Jack Domenech2, Arthur Govers1
1Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands.
来自Rhodococcus erythropolis的一种新型的Imine降解酶 (IRED),RytRedAm,在中性pH下有效催化降解氨化. 这种酶独特地利用NADPH和NADH共因子,扩大了合成氨酸生产的合成应用.
科学领域:
- 生物催化剂是一种生物催化剂.
- 酶学 是一种酶学.
- 有机化学 有机化学
背景情况:
- 降解性氨基化是一种关键的合成路径,用于奇拉胺.
- 氨基胺还原酶 (IREDs) 或还原氨基酶 (RedAms) 催化这种反应,但通常需要昂贵的NADPH和基本pH.
- 现有的IRED在辅因子灵活性和最佳反应条件方面存在局限性.
研究的目的:
- 发现和描述一种具有改善催化性能的新型Imine降解酶 (IRED).
- 探索这种新酶的合成潜力,以产生性氨基.
- 了解其独特的辅因子灵活性的结构基础.
主要方法:
- 发现和隔离来自Rhodococcus erythropolis (RytRedAm) 的IRED.
- 酶测试以确定基质范围,转化,酶选择性和辅因子利用率 (NADPH和NADH).
- 进行X射线晶体学以阐明酶的结构和活性部位.
主要成果:
- 在中性pH下,RytRedAm有效地催化各种碳基和氨基化合物的还原性氨基化 (>99%转化,99% ee).
- 与真菌RedAms相比,该酶表现出相反的立体选择性,产生了 (S) -enantiomer.
- RytRedAm表现出了显著的灵活性,利用NADPH和NADH具有不同的动力参数.
结论:
- RytRedAm代表了一种新的IRED子家族,能够进行依赖NADH的还原性氨基化.
- 它的中性pH活性和双辅助因子的使用为合成化学提供了显著的优势.
- 这一发现扩大了通过生物催化剂获取多样化胺的工具包.
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