在受伤后重新上皮化过程中,Irx1机制对口腔上皮基础干细胞可塑性
Dan Su1,2, Tadkamol Krongbaramee3,4, Samuel Swearson1,2
1Department of Anatomy and Cell Biology.
JCI insight
|January 9, 2025
概括
伊洛quois Homeobox 1 (IRX1) 蛋白质对于口腔组织的修复至关重要. 它在小鼠中的缺乏,通过影响细胞迁移和增殖,损害了牙伤口愈合.
科学领域:
- 口腔生物学 口腔生物学
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 口腔粘膜需要不断的再生来保持口腔健康.
- 牙基底细胞层中的干细胞对于组织修复至关重要.
- 伊洛quois Homeobox 1 (IRX1) 在平衡过程中存在于口腔上皮质和介质干细胞中.
研究的目的:
- 研究IRX1在口腔伤口愈合和牙再生中的作用.
- 阐明IRX1在修复过程中影响状细胞行为的分子机制.
主要方法:
- 使用了Irx1+/-异卵性 (Het) 鼠和野生类型 (WT) 的 littermates.
- 在受伤后3天 (dpi) 进行RNA测序 (RNA-Seq) 分析.
- 使用Krt14CreERT进行血统追踪实验.
主要成果:
- Irx1+/- Het小鼠表现出延迟的伤口关闭和缺陷的上皮再生.
- RNA-Seq在Het小鼠中揭示了表皮细胞迁移受损和状细胞相关基因表达的减少.
- 发现IRX1可以激活SOX9在口腔表皮中的增殖和EGF在口腔表皮中的迁移信号.
结论:
- IRX1在促进快速无痕的口腔伤口愈合方面发挥着至关重要的作用.
- 基底细胞层中的IRX1表达为有效的再生提供了牙.
- 缺少IRX1会在伤口修复过程中破坏上皮层分层,增殖和迁移.
更多相关视频
10:05Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
Published on: May 8, 2020
1.9K
08:14Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
Published on: May 1, 2014
15.2K
相关概念视频
01:12Renewal of Skin Epidermal Stem Cells
2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
01:19Clinical Applications of Epidermal Stem Cells
2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
01:23Renewal of Intestinal Stem Cells
2.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.5K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
01:19Overview of Regeneration and Repair
3.9K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
Regeneration
All animals have varying degrees of...
3.9K
01:28Phases of Wound Repair
5.9K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
5.9K