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在复杂的自然产品中提取生物活性成分的Noval选择性联体提取,使用固定式多目标磁珠

Chen Wang¹, Xueying Qin¹, Ying Wang¹

¹Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China.

Biomedical chromatography : BMC

|January 9, 2025

概括

冠状动脉支柱病显示出冠状动脉心脏病 (CHD) 的抗炎作用潜力. 研究人员使用先进的质谱仪确定了16种活性化合物,包括黄素和甲,证实了它们的药理动力学效应.

科学领域:

药理学药理学是指药理学的学科.
自然产品化学自然产品化学
生物化学生物化学

背景情况:

冠状动脉支柱菌 (Choerospondias axillaris) 是一种用于治疗冠状动脉心脏病 (CHD) 的传统药用草本.
它的治疗作用归因于其广泛的抗炎性质.
了解活性成分及其机制对于验证其药用至关重要.

研究的目的:

为了识别和分析Choerospondias axillaris中的活性抗炎成分.
调查其在治疗冠状动脉心脏病中的疗效的药理学基础.
开发一种有效的方法,用于丰富和分析复杂的自然产品中的生物活性化合物.

主要方法:

在磁性纳米颗粒上对循环氧化酶2 (COX-2) 和氧化酶5 (5-LOX) 酶进行固定,用于选择性联体提取.
使用超高性能液态色谱学与Q-Exactive Orbitrap双重质谱学 (UHPLC-Q-Exactive Orbitrap-MS/MS) 结合使用提取的组件的识别和分析.
评估16个已识别的成分的新陈代谢和药学动力学效应在老鼠诱导急性血静止.

主要成果:

成功提取和识别了16种活性成分,其中包括著名的黄类化合物,如黄素和甲素.
该研究证实了这些化合物的存在和活性,证明了它们在C. axillaris的抗炎作用中的潜在作用.
在大鼠中进行的代谢研究提供了在血液静止的背景下确定化合物的强大的药理动力学基础的证据.

结论:

开发的方法可以有效地丰富,分析和有效性评估复杂的天然产品中活性成分,如C. axillaris.
已识别的化合物,特别是黄素和甲,有助于该植物在治疗冠状动脉心脏病方面的已知疗效.
这项研究为Choerospondias axillaris的传统使用提供了科学基础,并为天然产品研究提供了有价值的方法.

关键词:

这种病名为Choerospondias axillaris.5 - 洛克斯 5 - 洛克斯在COX‐2中,二氧化碳排放量为2.在 UHPLC-Q-Exactive Orbitrap-MS/MS 中使用.急性血液静止模型目标渔业技术目标渔业技术

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