横向转移和重组燃料Ty4逆转移素在Saccharomyces中的进化
Jingxuan Chen1, David J Garfinkel2, Casey M Bergman1,3
1Institute of Bioinformatics, University of Georgia, 120 E. Green St., Athens, GA, USA.
Genome biology and evolution
|January 9, 2025
概括
水平转子转移 (HTT) 多次将Ty4逆转子子子家族引入了Saccharomyces酵母. 这种水平转移推动了通过再组合的新逆转移子基团的进化.
科学领域:
- 基因组学和进化生物学
- 分子进化分子进化
- 微生物真核细胞
背景情况:
- 水平转子转移 (HTT) 显著影响真核生物基因组进化.
- 大多数HTT事件的进化历史和精确影响尚未完全理解.
- 在密切相关的微生物真核生物中研究HTT至关重要,比如Saccharomyces酵母.
研究的目的:
- 阐明Saccharomyces属内的Ty4逆转移子家族转移的进化史和影响.
- 了解微生物真核生物中水平逆转移子转移的机制和后果.
主要方法:
- 分析了Ty4逆转移素亚家族的含量和整个Saccharomyces属的序列演变.
- 利用了短读和长读全基因组测序数据.
- 纳入了Saccharomyces mikatae菌株的新PacBio基因组组合.
主要成果:
- 确定了多个独立的HTT事件,将Ty4亚家族引入各种Saccharomyces系.
- 在S. mikatae和S. kudriavzevii中发现了新的Ty4基团,通过居民和水平转移子家族之间的再组合产生.
- 揭示了在Saccharomyces中复杂的Ty4亚家族模式的反复HTT和谱系特定的灭绝.
结论:
- 横向转子转移是一种动态力量,塑造了Saccharomyces中的逆转子转子含量.
- HTT促进了相关的逆转移素子家族的共存,通过再组合推动了新类的进化.
- 这项研究提供了Saccharomyces中的Ty4逆转移体的详细进化史,突出了HTT在基因组进化中的作用.
更多相关视频
相关概念视频
Overview of Transposition and Recombination
15.2K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.2K
DNA-only Transposons
14.3K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.3K
LTR Retrotransposons
17.3K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.3K
Non-LTR Retrotransposons
11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K
Types of Genetic Transfer Between Organisms
27.0K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
27.0K
Conservative Site-specific Recombination and Phase Variation
5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
5.9K


