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在SBR反应堆中,用化培养物去除安皮西林

Daniel Maturano-Carrera¹, Omar Oltehua-López¹, Flor de María Cuervo-López¹

¹Department of Biotechnology-CBS, Metropolitan Autonomous University Iztapalapa, Av. Ferrocarril San Rafael Atlixco 186, 09310, Mexico City, Mexico.

Applied biochemistry and biotechnology

|January 9, 2025

概括

化污泥通过生物吸收和生物转化,主要通过氨基单氧酶 (AMO) 酶,有效地从废水中去除安培 (AMP). 这表明了氨氧化细菌对抗生素污染水的生物修复的潜力.

科学领域:

环境微生物学环境微生物学
废水处理废水处理
生物修复是一种生物修复.

背景情况:

废水中的抗生素污染给环境带来了风险,推动了抗生素耐药性的发展.
化污泥表现出对清除氨和新出现的有机污染物,包括抗生素的代谢能力.
了解通过化培养去除抗生素的机制对于有效的废水处理策略至关重要.

研究的目的:

通过在测序批量反应堆 (SBR) 中化污泥来评估安皮西林 (AMP) 的去除能力.
研究生物吸收,生物转化和特定酶 (AMO,NOR,β-乳酸酶) 在AMP生物降解中的贡献.
评估AMP对化过程和污泥长期适应性的影响.

主要方法:

在使用化污泥的SBR系统中研究了AMP的去除.
在批量培养中使用了特定的抑制剂,以确定化酶和β-乳酸酶在AMP生物降解中的作用.
在整个操作过程中,对氧化能力和动力活性进行了监测.

主要成果:

在操作5小时内实现了100%的安比西林清除.
24%的AMP去除是由于生物吸收和76%的生物转化,而AMO酶对生物降解有95%的贡献.
保持了化能力,尽管AMP暂时影响了酸盐氧化;然而,随着时间的推移,污泥活动有所改善. 没有检测到β-乳酸酶活性.

结论:

化污泥具有显著的安培生物降解能力,主要由AMO酶介导.
氨氧化细菌的代谢能力为用抗生素污染的废水的生物修复提供了一个有希望的方法.
长期暴露于AMP并没有诱导β-乳糖酶活性,这突显了对现有的酶途径的依赖.

关键词:

阿姆皮西林是一种抗胰岛素.生物吸收生物吸收合成的生物降解.化污泥中的化污泥排序批量反应堆的排序

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