信号传输网络ACE2的解 通过使用整合性TMT蛋白质学和转录学来调节日本的自解
Jinghe Sun1, Tingting Yan1, Xiaoyan Wang1
1School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China.
Journal of agricultural and food chemistry
|January 9, 2025
概括
海黄的自解受血管转化酶2 (ACE2) 和NF-κB通路的调节. 激活ACE2可以防止亡,而抑制它则可以加速自分解.
科学领域:
- 海洋生物学 海洋生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 海 (Apostichopus japonicus) 的自解是一个复杂的过程.
- 了解底层的信号网络对于水产养殖和健康管理至关重要.
研究的目的:
- 为了阐明控制海自解的信号传导网络.
- 研究血管酶转化酶2 (ACE2) 在调节自解和亡中的作用.
主要方法:
- 协同质量标签 (TMT) 蛋白质组学和转录组学用于差异表达分析.
- 流细胞测量检测细胞亡.
- 西部涂抹和RT-PCR用于验证信号通路.
- 使用ACE 2激活剂 (diminazene, DIZE) 和抑制剂 (captopril, Capt) 的药理学操纵.
主要成果:
- 通过激活NF-κB通路,ACE 2激活剂 (DIZE) 维持了海黄的健康,并预防了细胞亡.
- ACE 2 抑制剂 (Capt) 通过 TNF 和 PI3K-Akt 信号传递加速了自解并启动了亡,这些信号会降低 NF-κB 的调节.
- 鉴定出Akt是ACE 2调节自解的关键下游信号蛋白.
- 治疗Akt抑制剂 (perifosine,KRX) 和DIZE减弱了自解和ROS水平.
结论:
- ACE 2-Akt-NF-κB轴在调节海自解中发挥着至关重要的作用.
- 针对ACE 2和下游途径提供了管理海黄健康和预防自解的潜在策略.
关键词:
2ACE 2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2ACE2亚波斯蒂科普斯 (Apostichopus japonicus) 是一个日本动物.灭症 (apoptosis) 是一种死亡的过程.自溶解是一种自溶的过程.这些机制的机制.相关概念视频
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