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狗尿细胞模型用于研究由尿病原性大肠杆菌 (Escherichia coli) 引起的细胞内细菌群体的发展

Jessica M Gilbertie^1,2, Breanna J Sheahan^2,3, Shelly L Vaden³

¹Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, United States of America.

|January 9, 2025

概括

狗体内由尿病原性大肠杆菌 (UPEC) 形成的细胞内细菌群体 (IBC) 与经常性尿路感染 (UTI) 有关. 这些IBC对常见的抗生素具有耐药性,这表明狗可以作为研究尿路感染的有价值模型.

科学领域:

兽医医学兽医医学兽医医学
微生物学微生物学
传染性疾病传染性疾病

背景情况:

尿路感染 (UTI) 在狗和人类中很常见,通常是由尿病原性大肠杆菌 (UPEC) 引起的.
复发的UPEC感染在两种物种中都带来了治疗挑战.
尿路细胞内的细胞内细菌群体 (IBC) 与人类的反复性尿路感染有关,但它们在犬类尿路感染中的作用尚未被探索.

研究的目的:

研究IBCs在犬类UPEC相关的尿路感染中的存在和作用.
为了确定狗的IBC形成是否与尿路感染复发和特定的UPEC基因相关.
为了评估犬类UPEC IBCs的抗生素耐受性.

主要方法:

在尿液和膀组织中识别出患有尿路感染的狗的IBCs.
在实验室中,IBCs的形成使用了初级犬类尿细胞和犬类UPEC分离物.
IBC形成与 fimH 基因和尿道感染复发的相关性分析.
对IBCs进行抗生素敏感性测试,以检测其对恩罗夫洛克萨,塞夫多克西姆和多克西环素的敏感性.

主要成果:

在犬类尿路感染病例中发现了IBC,而犬类UPEC分离物在体外形成了IBC.
IBC 形成与 fimH 基因存在相关.
来自复发性尿路感染的UPEC菌株表现出更高的IBC形成率.
狗的UPEC IBCs表现出耐受性对临床相关度的恩罗夫洛克萨, cefpodoxime 和多克西环素.

结论:

IBC形成是犬类UPEC尿路感染的特征,并与复发有关.
fimH基因与犬类IBC形成有关.
狗的UPEC IBC对关键抗生素具有耐药性,这解释了治疗方面的挑战.
狗代表了研究尿路感染IBC病理生理学的相关自然模型.

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