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相关概念视频

Affinity Chromatography01:03

Affinity Chromatography

525
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
525
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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相关实验视频

Updated: Jun 3, 2025

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
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Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

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利用先前的知识在mAb蛋白A染色学中进行过程参数分类.

Yinying Tao1, Adam Rauk1, Jinxin Gao1

  • 1Eli Lilly and Company, Indianapolis, IN 46285, USA.

Journal of chromatography. A
|January 9, 2025
PubMed
概括

利用蛋白质A染色体的广泛先验知识,可以简化单克隆抗体 (mAbs) 的净化. 这种方法证实过程参数不会显著影响关键质量属性,减少了对产品特定研究的广泛需求.

科学领域:

  • 生物制药制造业 生物制药制造业
  • 染色体学 染色体学 是一种染色学.
  • 抗体净化 抗体净化

背景情况:

  • 蛋白A (ProA) 亲和色谱对于净化单克隆抗体 (mAbs) 是至关重要的.
  • 对于 ProA 流程,从几十年的 mAb 开发中获得了广泛的先验知识.
  • 这种知识有助于简化流程开发,并尽量减少广泛的表征.

研究的目的:

  • 为蛋白质A染色学提供一个全面的先验知识包.
  • 为了整合十个分子的过程参数表征数据.
  • 为了证明利用历史数据对新治疗抗体的有效性.

主要方法:

  • 整合了来自十个分子的过程参数表征数据.
  • 进行了多项实验设计 (DOE) 研究.
  • 应用贝叶斯的层次模型来分析历史数据.

主要成果:

  • 没有观察到过程参数对平台范围内的关键质量属性的显著影响.
  • 贝叶斯模型证实了ProA平台范围内的微不足道的参数效应.
  • 历史数据包支持对新抗体的ProA过程参数进行分类.
关键词:
抗体是对抗体的一种.贝叶斯统计学 贝叶斯统计学之前的知识 之前的知识过程参数的表征 过程参数的表征蛋白质A染色体的染色学

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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

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相关实验视频

Last Updated: Jun 3, 2025

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
10:00

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

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结论:

  • 利用对蛋白质A染色体的先验知识包,简化了过程开发.
  • 产品特定的工艺特征可以通过使用历史数据来取代.
  • 这种方法得到了全球监管机构的积极欢迎.