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通过调节Bcl-2/Bax/Caspase-3/PARP通路,ACNO水凝可以增强糖尿病伤口愈合

Penghui Ye¹, Xingqian Wu¹, Rifang Gu²

¹College of Pharmacy, Zunyi Medical University, Zunyi 563006, China; Key Lab of the Basic Pharmacology of the Ministry of Education & Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563006, China.

International immunopharmacology

|January 9, 2025

概括

亚洲化物-氧化水凝 (ACNO) 通过增强细胞存活率和血管形成,促进糖尿病伤口愈合. 这种传统中医方法调节了关键的亡途径,为持久性伤害提供了一种新的治疗方法.

科学领域:

药理学药理学是指药理学的学科.
生物医学工程生物医学工程
传统中国医药传统中国医药

背景情况:

亚洲 (Centella asiatica) 是一种传统的中医药,用于治疗伤口.
亚洲化物-氧化水凝 (ACNO) 显示出糖尿病伤口 (DW) 治疗的潜力.
在DW治疗中ACNO的分子机制需要进一步研究.

研究的目的:

确定ACNO的抗亡目标,并阐明其在糖尿病伤口愈合中的作用机制.
评估ACNO水凝在促进糖尿病伤口关闭方面的有效性.
探索ACNO对细胞增殖,迁移和细胞亡的影响.

主要方法:

在患有糖尿病伤口的糖尿病 (db/db) 和非糖尿病 (db/m) 鼠标模型上局部应用ACNO.
通过摄影,组织学,分子对接,RT-qPCR和西式涂抹来评估伤口愈合.
使用高糖化HFF-1细胞进行体外研究以评估迁移,增殖和亡,并使用BCL-2抑制剂进行机械确认.

主要成果:

在糖尿病小鼠中,ACNO水凝显著促进了伤口上皮质化和血管生成.
ACNO增强了增殖和迁移,同时降低了高糖化HFF-1细胞中的亡.
ACNO调节了Bcl-2/Bax/Caspase-3/PARP信号级联,促进细胞生长和减少细胞死亡.

结论:

通过调节亡和促进血管生成,ACNO水凝有效地增强了糖尿病伤口愈合.
这项研究阐明了ACNO涉及Bcl-2/Bax/Caspase-3/PARP通路的机制.
这项研究突出了中国传统医学的潜力,用于治疗糖尿病伤害等复杂的伤口.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.亚洲的边缘 Bcl-2 的使用方法.糖尿病病人的伤口.在PARP中,PARP是PARP.

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