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miR-92a-3p通过侵入性秋季军Spodoptera frugiperda的核子生成来调节卵子受精

Jyoti Pathak¹, Selva Babu Selvamani¹, Subhi Srivastava¹

¹Division of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, P. Bag No: 2491, H.A. Farm Post, Bellary Road, Hebbal, Bengaluru - 560024, India.

International journal of biological macromolecules

|January 9, 2025

概括

表观遗传分子机制 (EMM) 控制了Spodoptera frugiperda中的繁殖. 一种特定的微RNA (miRNA) 和其向基因BOP1对于卵子生成和卵子受精至关重要,为害虫控制提供了新的向.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
生殖生物学生殖生物学

背景情况:

表观遗传分子机制 (EMM) 对于理解昆虫繁殖至关重要.
通过阐明这些机制,可以改进Spodoptera frugiperda的控制策略.

研究的目的:

为了研究微RNAs (miRNAs) 在Spodoptera frugiperda繁殖中的作用.
为了确定关键的miRNA-目标相互作用调节 oogenesis 和受精.

主要方法:

在雄性和雌性S. frugiperda中对miRNA的差异表达分析.
预测分析用于识别miRNA目标基因.
蛋白与蛋白相互作用 (PPI) 网络分析以确定枢纽基因.
实验验证miRNA和目标基因功能的验证.

主要成果:

在处女雌性和雄性中鉴定出11种差异表达的miRNA.
预测了426个向基因,突出了 oogenesis 中的核生成.
鉴定了miR-92a-3p,它准了枢纽基因CG5033 (BOP1),这对于rRNA成熟至关重要.
实验证据显示,miR-92a-3p的耗尽或BOP1的敲击导致不育卵.

结论:

在S. frugiperda中,miR-92a-3p - BOP1相互作用对生殖和受精至关重要.
这一监管轴为开发新型害虫控制策略提供了潜在的目标.

关键词:

生殖生殖的繁殖它们是花鸟 (Spodoptera frugiperda) 的种类.微RNAs 是一个微型RNA.

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