Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Long-Chain Fatty Acid Oxidation Disorder Genes: A Comprehensive Genetic Database of LC-FAOD Variants, Genotypes, and Phenotypes.

Human mutation·2026
Same author

CDK1 and CEP97 cooperatively control centriole length to orchestrate ciliogenesis and developmental patterning.

Genes & development·2026
Same author

Metabolomic assessment reveals depletion of amino acids and energy metabolites in skeletal muscle after ischemic acute kidney injury in mice.

Scientific reports·2026
Same author

Extracellular matrix proteolysis maintains synapse plasticity during brain development.

Nature neuroscience·2025
Same author

Identification of the peptide Vulnusin, a wound signal that mediates mechanical-injury-induced nociception in Drosophila.

Neuron·2025
Same author

Proteomic characterization of type I collagen N-terminal crosslinked peptides.

Matrix biology plus·2025

相关实验视频

Updated: Jun 3, 2025

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
08:37

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

2.2K

使用蛋白质探测器提高糖标识的深度和可靠性.

Robert J Chalkley1, Peter R Baker1

  • 1Department of Pharmaceutical Chemistry, University of California, San Francisco, USA.

Molecular & cellular proteomics : MCP
|January 9, 2025
PubMed
概括

蛋白质探测器软件增强了N-glycopeptide数据分析,比其他工具识别了更多的糖形. 它还通过考虑甘氨酸添加剂来纠正以前的错误分配.

科学领域:

  • 生物化学 生物化学
  • 蛋白质组学是指蛋白质组学.
  • 格莱科米克斯 (Glycomics) 是一个有趣的药物.

背景情况:

  • 蛋白质糖化是一种常见的,多样化的翻译后修改,影响蛋白质的功能,稳定性和疾病相关性.
  • 虽然蛋白质组分析方法很先进,但糖数据分析仍然具有挑战性.
  • 目前用于分析N链 glycopeptide 的软件存在局限性.

研究的目的:

  • 介绍蛋白质探测器对N-糖的数据分析的进展.
  • 为了比较蛋白质探测器与其他糖类分析软件的性能.
  • 评估考虑甘氨酸添加物对甘氨酸分配准确性的影响.

主要方法:

  • 开发和应用蛋白质探测器软件用于N-糖分析.
  • 用Protein Prospector和其他软件对糖标识结果进行比较分析.
  • 对甘氨酸添加剂对数据解释的影响的评估.

主要成果:

  • 与竞争软件相比,蛋白质勘探器识别出了显著更高数量的葡萄糖形式.
  • 考虑糖添加物可以纠正以前不正确的糖分配.
  • 蛋白质探测器提供了对欧米级N-链 glycopeptide 分析的改进功能.
关键词:
蛋白质探矿机是一种蛋白质探矿机.数据库搜索 搜索数据库搜索葡萄糖的ID是什么?葡萄糖类是葡萄糖类中的一种.葡萄糖蛋白质组学药物

更多相关视频

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

14.9K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

925

相关实验视频

Last Updated: Jun 3, 2025

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
08:37

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

2.2K
A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

14.9K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

925

结论:

  • 蛋白质探测器为N-葡萄糖数据分析提供了增强的功能.
  • 在分析中将甘氨酸添加物纳入分析中对于准确的甘氨酸鉴定至关重要.
  • 这些进展解决了糖酸数据分析中的一个关键挑战.