通过代谢和发酵工程通过Komagataella phaffii进行微生物阿斯丁合成
Jingnan Wang1, Weixu Ma1, Wenqi Ma1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, P.R. China.
Journal of agricultural and food chemistry
|January 9, 2025
概括
强大的抗氧化剂胡卜素阿斯塔克桑丁的微生物生产在Komagataella phaffii (K. phaffii) 中得到了优化. 这项研究通过代谢工程和发酵优化实现了创纪录的716.13 mg/L的阿斯塔山丁产量.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物发酵 微生物发酵
背景情况:
- 阿斯塔桑丁是一种强大的抗氧化类胡卜素,在医疗保健,化品和水产养殖中具有多种应用.
- 对天然产品的需求不断增长,推动了对微生物阿斯塔克桑生产的兴趣.
- 在Komagataella phaffii (K. phaffii) 中,阿斯塔桑合成途径的代谢构造是研究的一个关键领域.
研究的目的:
- 为了代谢工程 K. phaffii 有效的阿斯塔克桑生物合成.
- 为了优化发酵条件以最大限度地提高阿斯塔山丁产量.
- 在K. phaffii.中建立阿斯塔克桑生产的新纪录.
主要方法:
- 在K. phaffii.中构建阿斯塔丁合成的代谢途径.
- 探索促进剂组合和酶组合策略.
- 优化发酵参数 (温度,营养素,添加剂) 和NADPH供应.
- 在5L生物反应器中进行料批发发酵.
主要成果:
- 在K. phaffii.中成功构建了阿斯塔山丁合成途径.
- 确定了最佳的促进剂和酶组合,以提高产量.
- 确定理想的发酵条件,包括营养度和添加剂.
- 取得了716.13毫克/升的阿斯塔克桑纪录产量.
结论:
- 代谢工程和发酵优化显著提高了K. phaffii. 的阿斯塔山丁生产.
- 开发的K. phaffii菌株和工艺代表了微生物阿斯塔克桑合成的突破.
- 这项研究为大规模,具有成本效益的微生物生产阿斯塔克桑丁提供了一个强大的平台.
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