相关实验视频

Updated: Jun 3, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

在编排棕色和白色脂肪细胞转录组和系统代谢方面,m6A的不同作用

Ling Xiao¹, Dario F De Jesus¹, Cheng-Wei Ju²

¹Section of Islet Cell and Regenerative Biology, Joslin Diabetes Center; Department of Medicine, BIDMC; Harvard Stem Cell Institute, Harvard Medical School, Boston, MA, USA.

Nature communications

|January 9, 2025

概括

由METTL14规范的N6-甲基氨酸 (m6A) 修饰在棕色和白色脂肪中具有不同的作用. METTL14通过控制脂肪细胞命运和代谢途径,影响胰岛素敏感性.

科学领域:

生物化学生物化学
分子生物学分子生物学
代谢过程中的代谢.

背景情况:

N6-甲基氨酸 (m6A) 是一种流行的mRNA修饰,但其组织特异性功能尚未完全理解.
METTL14是m6A甲基化中的关键酶,其作用在脂肪组织亚型中需要阐明.

研究的目的:

研究METTL14在棕色脂肪组织 (BAT) 和白色脂肪组织 (WAT) 中细胞类型特定的调节作用.
确定METTL14对不同脂肪储存中的代谢结果和胰岛素敏感性的影响.

主要方法:

对人类和具有胰岛素抵抗性的小鼠在BAT和WAT中的METTL14表达的比较分析.
在BAT和WAT中生成和分析Mettl14-knockout模型.
整合m6A-seq和RNA-seq以确定差异调节的途径.
在稳定的METTL14-knockout人类棕色和白色脂肪细胞 (hBAs/hWAs) 中的验证.

主要成果:

在BAT和WAT中,METTL14表达与胰岛素敏感性相关.
在BAT中Mettl14-knockout通过前列腺素分泌增强胰岛素敏感性.
在WAT中Mettl14-Knockout诱导脂肪细胞亡和胰岛素抵抗.
在BAT中,METTL14缺陷对前列腺素生物合成和在WAT中对亡途径进行上调,影响特定的mRNA标.

结论:

METTL14对脂肪组织代谢和胰岛素敏感性进行细胞类型特定的控制.
通过METTL14的m6A修饰会影响脂肪细胞的命运和全身代谢健康.
针对METTL14介导的m6A通路可能为代谢疾病提供治疗策略.

更多相关视频

Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation

Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation

Published on: January 5, 2024

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Regulation of Metabolism

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...