揭示了通过内分泌网膜压力相关的基因表达重复流产的分子机制
Xiaodan Yin1, Wei Yang1, Mingwei Xin1
1Department of TCM, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, 100026, China.
Scientific reports
|January 9, 2025
概括
细胞内膜网膜应激 (ERS) 与反复流产 (RM) 有关. 研究人员确定了与ERS相关的基因作为诊断RM的潜在生物标志物,显示出高预测准确度.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 基因组学就是基因组学.
背景情况:
- 经常性流产 (RM) 影响全球许多夫妇,其分子原因尚不清楚.
- 新出现的证据表明,内等质网膜应激 (ERS) 在RM病变发生过程中起着作用.
- 了解RM中ERS的遗传基础对于开发诊断工具至关重要.
研究的目的:
- 研究与RM和ERS相关的基因.
- 使用ERS相关基因开发RM预测的诊断模型.
- 为了确定早期检测RM的潜在生物标志物.
主要方法:
- 通过R包GEOquery.使用的基因表达综合 (GEO) 数据.
- 应用批次校正,正常化和差异基因表达分析 (limma).
- 使用基因本体学和KEGG通路分析识别了与ERS相关的差异表达基因 (ERSRGs).
- 开发了诊断模型 (逻辑回归,SVM,LASSO) 并进行了免疫透分析.
主要成果:
- 在RM中鉴定了1395个差异表达基因 (DEG),包括17个ERSRG.
- KEAP1和YIPF5显示了高的诊断准确性 (AUC>0.9).
- 与ERS相关的DEG参与了细胞对ERS的反应,蛋白质加工和亡.
- 诊断模型实现了强大的预测性能 (AUC > 0.9).
结论:
- 在RM和ERS反应之间存在分子联系.
- 已识别的ERSRG,如KEAP1和YIPF5,显示出作为RM诊断生物标志物的前景.
- 开发的诊断模型为RM提供了强大的预测能力.
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