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感染ISKNV的ISKNV和Siniperca chuatsi细胞中的m6A甲基化的转录组范围的动态

Qijin Miao^1,2, Jing Jiang¹, Siyou Huang¹

¹State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology, Guangdong Provincial Key Laboratory of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.

|January 9, 2025

概括

这项研究探讨了N6-甲基氨酸 (m⁶A) RNA甲基化在传染性脏亡病毒 (ISKNV) 感染. 它揭示了M6A修改对关键基因的影响,为ISKNV病原性提供了洞察力.

科学领域:

病毒学病毒学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

感染性脏亡病毒 (ISKNV) 是水产养殖的重大威胁.
N6-甲基氨酸 (m6A) RNA甲基化是病毒感染中的关键表观遗传调节剂.
目前尚不清楚m6A在ISKNV病原性中的作用.

研究的目的:

为了研究ISKNV感染细胞中的全基因组m6A甲基化概况和基因表达变化.
通过m6A在ISKNV感染期间的修饰来调节的关键基因和通路的识别.
建立一个基础,以了解m⁶A在ISKNV病变发生中的作用.

主要方法:

使用甲基化RNA免疫沉降测序 (MeRIP-seq) 和RNA测序 (RNA-seq).
感染和未感染的MFF-1细胞之间的m6A甲基化和基因表达的比较分析.
进行了功能丰富和共同表达分析.

主要成果:

超过4361种不同的m6A甲基化mRNA被确定,主要是在编码序列中.
m6 A相关基因在免疫反应,细胞死亡和基因表达途径中得到丰富.
同表达分析突出显示了Hippo,ErbB和JAK-STAT通路.
在诸如血管内皮生长因子和E3泛素酶等基因中观察到显著的m6A修饰.

结论:

这项研究提供了一个全面的m⁶在ISKNV感染细胞中的表达特征.
在ISKNV感染期间,一种修饰在调节基因表达方面起着显著的作用.
这些发现为未来研究中A介导的ISKNV病原性提供了基础.

关键词:

锡尼珀卡 (Siniperca) 是一个小岛屿.这是ISKNVV.在MFF-1单元格中,请输入MFF-1单元格.这就是MeRIP-seqq.这是一种N6-甲基氨酸.

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