使用Parageobacillus thermoglucosidasius DSM 6285进行批量和半连续发酵,用于H2生产
Magda S Ardila1, Habibu Aliyu2, Pieter de Maayer3
1Section II: Electrobiotechnology, Institute of Process Engineering in Life Science, Karlsruhe Institute of Technology, 76131, Karlsruhe, Germany.
Biotechnology for biofuels and bioproducts
|January 9, 2025
概括
将葡萄糖半连续给Parageobacillus thermoglucosidasius显著增加了气的产生. 优化食间隔是实现生物平台稳定和增加产量的关键.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
背景情况:
- 帕拉基奥巴西勒斯 (Parageobacillus thermoglucosidasius) 通过水气转移反应产生 (H2).
- 之前的化研究使用了批量发酵.
- 这项研究研究了半连续养,以改善H2生产.
研究的目的:
- 评估半连续养策略对P. thermoglucosidasius. 的气生产的影响.
- 为了比较不同养方案下的气生产率和一氧化碳脱酶 (CODH) 活性.
- 为了确定稳定的生物生成的最佳食间隔.
主要方法:
- 使用含葡萄糖介质进行了P. thermoglucosidasius的批量和半连续发酵.
- 实施24小时和48小时间隔的半连续养.
- 监测H2生产,CODH活性和代谢物变化.
主要成果:
- 与批次发酵 (0.14 mmol min-1) 相比,半连续养实现了更高的最大H2生产率 (0.3 mmol min-1).
- 每天养导致7天稳定的H2产生,而48小时的养显示出很高的变化.
- CODH活性与H2产生相关,在48小时养后达到峰值,但在24小时养后保持稳定.
结论:
- 半连续用葡萄糖食对于P. thermoglucosidasius. 的稳定气生产至关重要.
- 半连续方法比批量发酵提高了46%的H2生产率,这表明生物平台的潜力.
- 优化食间隔对于最大化和稳定产量至关重要.
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