临床部署的电免疫传感器,包括生物活性玻璃-MWCNT,用于检测人类血清样本中的性酸酶
Darshna1, Indrani Nandi1, Pradeep Srivastava1
1School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
ACS applied bio materials
|January 10, 2025
概括
使用生物活性玻璃和碳纳米管的新型生物传感器检测血清中的性酸酶 (ALP). 这种无标签的电化学阻抗光谱法为临床骨疾病评估提供了高灵敏度和选择性.
科学领域:
- 生物材料科学 生物材料科学
- 生物传感器技术技术
- 分析化学 分析化学
背景情况:
- 骨是一种动态组织,对储存,造血和结构支持至关重要.
- 由骨质母细胞分泌的酸酶 (ALP) 是骨代谢和疾病的关键生物标志物.
- 目前用于ALP检测的方法可能需要标签或缺乏足够的灵敏度用于临床诊断.
研究的目的:
- 开发一种新的,无标签的生物传感器,用于检测性酸酶 (ALP).
- 使用生物活性玻璃 (BG) 和多壁碳纳米管 (MWCNT) 的复合探头进行增强检测.
- 评估生物传感器在生物样本中的分析性能和选择性.
主要方法:
- 使用生物活性玻璃 (BG) 和多壁碳纳米管 (MWCNT) 制造复合探头.
- 通过物理方法和电化学分析对生物传感器探针进行表征.
- 使用电化学阻抗光谱 (EIS) 评估分析性能,包括检测极限 (LOD) 和线性动态范围 (LDR).
- 对血清干扰化学物质的选择性评估和人类和胎儿牛血清样本的验证.
主要成果:
- BG/MWCNT复合生物传感器通过EIS证明了ALP的无标签检测.
- 达到2.29±0.35U/L的检测极限 (LOD) 和25-600U/L的线性动态范围 (LDR).
- 对ALP表现出高选择性,选择性系数 (Ksel) <0.06.
- 在人血清和胎儿牛血清样本中成功检测到ALP.
结论:
- 开发的BG/MWCNT复合生物传感器是敏感和选择性ALP检测的一个有前途的工具.
- 这种无标签的电化学方法为临床评估骨相关疾病提供了可行的替代方案.
- 生物传感器的性能特性适用于常规的临床诊断和生物样本分析.
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