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慢性暴露于低剂量的MC-LR会通过PI3K/AKT/mTOR通路在小鼠中诱导乳腺炎症

Mingjie Yang¹, Shuilin Zheng², Wen Zeng³

¹School of Public Health, Hengyang Medical School, University of South China, Hengyang, China.

Journal of toxicology and environmental health. Part A

|January 10, 2025

概括

在小鼠中,在饮用水中慢性暴露于低剂量的微囊素-LR (MC-LR) 引起了乳腺炎症和损伤. 这涉及PI3K/AKT/mTOR信号通路的激活,突出显示了蓝藻毒素的潜在风险.

科学领域:

环境毒理学环境毒理学
胃肠病学胃肠病学
细胞生物学细胞生物学

背景情况:

在全球范围内,由于气候变化和肥胖化,蓝菌的繁殖量正在增加.
有害的蓝藻细菌会产生诸如microcystin-LR (MC-LR) 等毒素,这些毒素会损害器官,特别是肠道.
以前对MC-LR肠毒性的研究主要集中在结直肠,对小肠的数据有限.

研究的目的:

为了研究慢性低剂量MC-LR暴露对小鼠大肠的影响.
探索MC-LR诱导的乳腺炎症和损伤的潜在机制.

主要方法:

小鼠在饮用水中暴露在120μg/L的MC-LR中,持续9个月.
进行了对乳腺组织的组织病理学分析.
量化PCR (qPCR) 和西斑被用于评估炎症因素和信号通路的基因和蛋白质表达水平.

主要成果:

慢性低剂量MC-LR暴露导致肠道上皮细胞失调,淋巴细胞透,并扰乱了大肠中的密室.
促炎性细胞因子 (IL-6,IL-17,IL-18,IFN-γ) 的mRNA水平显著升高.
增加了p-PI3K,p-AKT和p-mTOR的蛋白质水平,表明PI3K/AKT/mTOR通路的激活.

结论:

MC-LR会诱导乳腺炎症和组织病理学损伤.
PI3K/AKT/mTOR信号通路参与了MC-LR诱导的阴茎损伤的发病过程.
这项研究强调了与饮用水中低水平的蓝菌毒素的长期暴露相关的潜在健康风险.

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