单核RNA测序揭示了猪骨肌肉不同生长阶段的细胞转录组特征
Ziyu Chen1,2, Xiaoqian Wu1, Dongbin Zheng2
1Chongqing Academy of Animal Science, Chongqing 402460, China.
Cells
|January 10, 2025
概括
猪肉肌肉随着年龄的增长显示出明显的细胞变化,揭示了导致纤维化和影响肉质的纤维/基原始细胞. 这项研究使用单核RNA测序来分析这些与年龄相关的细胞差异.
科学领域:
- 分子生物学分子生物学
- 动物科学动物科学
- 基因组学就是基因组学.
背景情况:
- 猪背肌 (LDM) 对于猪肉生产至关重要.
- 肉质受肌肉纤维成分和肌内脂肪 (IMF) 的影响.
- 骨肌肉表现出异质性,表型变异与细胞和转录差异有关.
研究的目的:
- 调查不同生长阶段的猪LDM细胞和转录特征 (170对245天).
- 确定细胞类型及其比例,重点关注纤维/基原始细胞 (FAP) 和脂肪细胞.
- 了解与猪骨肌肉衰老相关的分化轨迹和细胞-细胞通信.
主要方法:
- 单核RNA测序 (snRNA-seq) 在170日和245日从大白猪的LDM上进行.
- 分析了细胞组成,识别了肌纤维,FAP,肌肉卫星细胞 (MUSC) 和其他细胞类型.
- 在两个生长阶段之间比较了转录特征和细胞与细胞相互作用.
主要成果:
- 通过snRNA-seq识别了12个细胞群,在两个年龄组之间细胞类型的比例和状态不同.
- 在245天大的猪中观察到更高比例的FAP细胞,显示出与其他细胞类型的通信增加.
- 确定了三个FAP亚类 (FAPs_COL3A1+,FAPs_PDE4D+,FAPs_EBF1+) 和两个脂肪细胞亚类 (Ad_PDE4D+,Ad_DGAT2+),具有不同的与年龄相关的流行和分化途径.
结论:
- 猪LDM在170至245天的年龄之间表现出显著的转录和细胞差异.
- 老猪中的FAP细胞显示出纤维化差异化增加,反映了人类骨肌肉与年龄相关的变化.
- 该研究提供了对影响猪肉质量和潜在的骨肌肉疾病的细胞机制的见解.
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