广泛的SIV中和抗体的隔离和结构揭示了近接螺旋式MPER表位,该表位被 rhesus多捐赠者类识别出来
Jason Gorman1, Renguang Du2, Yen-Ting Lai2
1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA.
Cell reports
|January 10, 2025
概括
研究人员确定了广泛中和抗体 (bnAbs),向的SIV膜近端外部区域 (MPER). 这些SIV bnAbs识别了与HIV-1疫苗试验中发现的类似表位.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 结构生物学 结构生物学
背景情况:
- 艾滋病毒-1包裹的膜近端外部区域 (MPER) 是广泛中和抗体 (bnAbs) 的关键目标.
- 疫苗引起的MPER导向抗体在人类临床试验中显示出有前途.
- 在SIV等相关病毒中识别类似的抗体标对于疫苗开发至关重要.
研究的目的:
- 为了确定广泛中和抗体 (bnAbs) 向MPER在类免疫缺陷病毒 (SIV) 感染的 rhesus.
- 描述这些SIV MPER指导的bnAbs识别的表位,并将其与HIV-1目标进行比较.
主要方法:
- 从受感染的身上分离和描述SIV MPER导向的bnAbs.
- 对SIV菌株组的抗体宽度和强度的评估.
- 确定bnAbs与SIV MPER的复合体中的晶体结构.
主要成果:
- 分离了四个SIV MPER导向的bnAbs系,在20个菌株的SIV面板中显示出高达90%的宽度.
- 晶体结构揭示了这些SIV bnAbs在N端MPER区域结合螺旋性表皮质.
- 这种表位类似于人类疫苗引起的HIV-1抗体所准的表位.
结论:
- 一个流行且可重复的SIV bnAbs类针对MPER中的一个保存的螺旋性表位.
- 这一发现表明,在SIV和HIV-1之间存在保存的抗体标,这与疫苗设计有关.
- 鉴定到的表位代表了开发针对SIV和HIV-1的有效疫苗的有希望的目标.
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