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血管系统的皮肤集成电遗传调节,以加速伤口愈合

Preetam Guha Ray^1,2, Ragavi Rajasekaran², Bitan Pratihar³

¹ETH Zurich, Department of Biosystems Science and Engineering, Klingelbergstrasse 48, Basel, CH-4056, Switzerland.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|January 10, 2025

概括

一种新的生物相容植入物,VOLT,使用直流 (DC) 电压来刺激工程细胞释放血管内皮生长因子A (VEGFA). 这促进了血管的生长,并加速了老鼠的伤口愈合.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
再生医学是一种再生医学.

背景情况:

新血管化对于医疗植入物和深层伤口愈合中的工程细胞活力至关重要.
促进血管化的现有策略往往需要复杂的方法或侵入性程序.

研究的目的:

开发一个生物相容,电导3D平台 (VOLT) 用于电压控制的基于细胞的疗法.
通过直接电流 (DC) 刺激,设计具有可以分泌血管内皮生长因子A (VEGFA) 的基因电路的细胞.
评估VOLT-VEGFA系统在促进新血管和伤口愈合方面的有效性.

主要方法:

通过3D打印和电,制造一个3D蜂波罗-多 (PCL) 矩阵 (VOLT).
设计具有直流电压控制,反应性氧物种 (ROS) 感应基因电路的细胞,用于VEGFA分泌.
在体内测试,使用小鼠皮下伤口愈合模型,每天对VOLT-VEGFA植入物进行电刺激.

主要成果:

对VOLT-VEGFA植入物的电刺激 (5V,20秒/天) 诱导了向的VEGFA分泌.
在受刺激的伤口中观察到显著加快的新血管化和颗粒组织形成.
在刺激组中,在15天内发生了更快的伤口关闭,完整的再上皮质化和皮肤再生.

结论:

该VOLT平台可简单,非细胞毒性,电压控制诱导从工程细胞治疗性蛋白质分泌.
沃尔特-维格法系统有效地促进新血管化,加速深层伤口的愈合.
这项技术对精准医学应用具有前景,包括增强植入物活力和再生疗法.

关键词:

电遗传学电遗传学基因交换机基因交换机基因交换机基因工程是基因工程,是基因工程.合成生物学合成生物学伤口愈合伤口愈合

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