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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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相关实验视频

Updated: Jun 3, 2025

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Published on: January 18, 2017

在植物根中的超分辨率膨胀显微镜.

Michelle Gallei¹, Sven Truckenbrodt¹, Caroline Kreuzinger¹

¹Institute of Science and Technology Austria, Am Campus 1, Klosterneuburg 3400, Austria.

|January 10, 2025

概括

扩展显微镜 (ExM) 通过物理扩展组织来提高植物的成像分辨率. 这种技术,PlantEx,揭示了Arabidopsis根的复杂细胞结构,克服了以前的限制.

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A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

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RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

相关实验视频

Last Updated: Jun 3, 2025

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Published on: January 18, 2017

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Published on: May 17, 2017

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

科学领域:

植物细胞生物学植物细胞生物学
显微镜技术的使用方法
分子成像学分子成像学

背景情况:

超分辨率显微镜提供超越光学衍射极限的增强空间分辨率.
在植物中广泛采用受到具有挑战性的光学特性和组织刚性的限制.
扩展显微镜 (ExM) 通过物理样本扩展来提高有效分辨率.

研究的目的:

开发和应用整个安装的ExM,用于Arabidopsis thaliana根组织 (PlantEx).
为了克服植物组织的机械约束,用于ExM应用.
为了实现显著的分辨率增加,在完整的植物组织中可视化亚细胞结构.

主要方法:

整体扩展显微镜 (ExM) 应用于阿拉比多普西斯·塔利亚纳根组织 (PlantEx).
整合PlantEx与刺激排放消耗 (STED) 显微镜.
微管细胞骨架组织和亚细胞器官相互作用的分析.

主要成果:

在Arabidopsis根中,PlantEx比传统显微镜提高了分辨率4倍.
微管细胞骨组织和细胞成分相互作用的详细可视化.
在完整的组织中,纳米尺度分辨率成像COPI涂层囊泡与戈尔吉仪器相关.

结论:

扩展显微镜是一种可行的技术,用于提高Arabidopsis根标本的成像分辨率.
植物Ex可以对植物细胞结构和分子组织进行详细的研究.
这项研究证明了ExM在促进植物细胞生物学研究方面的潜力.