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被用于乳酸消费和SCFA生产,主要是人类肠道微生物Eubacterium limosum

Saisuki Putumbaka¹, Gerrit J Schut¹, Michael P Thorgersen¹

¹Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 30602.

Proceedings of the National Academy of Sciences of the United States of America

|January 10, 2025

概括

欧细菌 (Eubacterium limosum) 利用乳酸产生有益的短链脂肪酸 (SCFA),通过偏好而不是酸盐. 这突出了的.

科学领域:

微生物学微生物学
人类肠道微生物组
代谢工程是代谢工程.

背景情况:

利莫菌 (Eubacterium limosum) 是人类关键的肠道微生物,产生促进健康的短链脂肪酸 (SCFA).
乳酸是肠道中SCFA的重要前体,但其通过E. limosum的代谢以前没有被描述.
了解E. limosum的乳酸利用对于了解肠道健康和SCFA生产至关重要.

研究的目的:

研究Eubacterium limosum在SCFA生产中使用乳酸盐的情况.
确定关键的酶,调节机制,以及参与乳酸代谢的辅因子依赖性.
探索作为人类肠道微生物群中的微营养素的潜在作用.

主要方法:

在乳酸和葡萄糖介质上培养E. limosum,与酸盐和酸盐的差异补充.
在不同的生长条件下进行基因表达分析 (特定基因的上调).
酶特性 (WOR1基质特异性) 和代谢途径重建.

主要成果:

在乳酸盐上生长时,E. limosum优先使用酸盐而不是酸盐,产生酸盐和丁酸盐.
编码依赖酶 (WOR1,FDH) 和乳酸代谢途径 (LCT,Propanediol) 的基因在乳酸上升调.
乳酸代谢由LctR,Rex和CcpA调节,Rex控制储存和Tuco生物合成.

结论:

E. limosum的乳酸转化为SCFA是一个高度依赖的过程.
WOR1可能会在乳酸降解过程中产生的乙甲中解毒.
可能是一个被低估的微量营养素,对人类肠道微生物组的功能和整体健康至关重要.

关键词:

黄油酸盐黄油酸盐是一种电子二分化是什么意思我们的肠道微生物组.金属酶是一种金属酶.

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