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Updated: May 12, 2026

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

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高性能抗体的绑定模式引导开发,针对特定位点的翻译后修饰

Mariapia Riso1, Rohan N Shah2,3, Akiko Koide1,4

  • 1Laura and Isaac Perlmutter Cancer Center, New York University Langone Health, New York, NY 10016.

Proceedings of the National Academy of Sciences of the United States of America
|January 10, 2025
PubMed
概括

研究人员开发了一个新平台,用于创建针对蛋白质翻译后修饰 (PTMs) 的高特异性重组抗体. 这种抗原结合方法确保了分子定义和可再生的抗体,提高了生物医学研究中的可重复性.

关键词:
亲和反应剂 亲和反应剂表观遗传学是指表观遗传学.基质子的修改 基质子的修改蛋白质工程工程 蛋白质工程信号传输 信号传输

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Improving Translational Accuracy02:07

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Leaky Scanning02:28

Leaky Scanning

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Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

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