阴道的性别和温度独立地影响Trachemys scripta中的生殖细胞分化和介质性进展
Talia Hatkevich1, Boris M Tezak1,2, Rafael D Acemel3,4
1Department of Cell Biology, Duke University Medical Center, Durham, NC 27701.
概括
温度直接影响乌的生殖细胞分化,影响变的启动和进展,不论性腺的性别. 这揭示了温度在生殖细胞发育中的关键作用,超出了温度依赖性性别决定 (TSD) 物种的体质影响.
科学领域:
- 发展生物学 发展生物学
- 生殖生物学 生殖生物学
- 遗传学 是一个遗传学.
背景情况:
- 在遗传性决定 (GSD) 系统中,体性决定了生殖细胞的命运.
- 与海一样,温度依赖性性别确定系统 (TSD) 提供了替代性的性别确定机制.
- 在红耳滑 (Trachemys scripta) 中,温度会影响生殖细胞数量.
研究的目的:
- 调查温度是否直接影响TSD物种的生殖细胞分化和性别认同.
- 为了将温度与性命分离,以隔离温度对生殖细胞的直接影响.
主要方法:
- 在男性促进温度 (MPT) 上化Trachemys scripta的蛋,并用17β-雌二醇 (E2) 治疗以诱导卵巢.
- 进行了 meiotic 传播分析,以检查胚胎细胞通过半变化的进展.
- 使用单细胞转录组分析来评估生殖细胞的基因表达特征.
主要成果:
- 卵巢中的生殖细胞在女性促进温度 (FPT) 发育时启动了半衰期,并通过前期I进行了进展.
- 在MPT中发育的E2诱导卵巢中的生殖细胞启动了化,但未能表现出突触.
- 单细胞转录组数据支持温度对生殖细胞分化的直接影响.
结论:
- 在这种TSD物种中,温度直接影响生殖细胞的性分化,独立于淋巴体 soma.
- 并非所有半球变异事件都处于体质控制之下,特别是在TSD的物种中.
- 温度在调节生殖细胞介质进展方面发挥着直接作用.
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