删除金属载体Zip14可以减少小鼠小肠上皮细胞中的主要基因相容性复合体II表达
Felix R Jimenez-Rondan1, Courtney H Ruggiero1, Alberto Riva2
1Center for Nutritional Sciences, Food Science and Human Nutrition Department, College of Agricultural and Life Sciences, University of Florida, Gainesville, FL 32611.
概括
载体Zip14的丧失通过减少主要基因相容性复合物II类 (MHCII) 表达而损害肠道免疫力. 补充会恢复MHCII水平和免疫功能,防止肠道炎症.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 营养科学 营养科学
背景情况:
- 饮食中缺乏会影响全球免疫力.
- 输送器调节的平衡.
- Zip14 (Slc39a14) 删除会导致肠道炎症.
研究的目的:
- 研究Zip14在肠道免疫中的作用.
- 确定将Zip14损失与炎症联系起来的分子机制.
- 评估补充剂对MHCII表达和炎症的影响.
主要方法:
- 净化肠道上皮细胞 (IEC) 的RNA测序.
- 定量PCR (qPCR),西部抹黑和免疫组织化学.
- 光谱测量,染色体免疫沉 (ChIP) 和ATAC-测序 (ATAC-seq). 这两种测序是最重要的.
主要成果:
- 删除Zip14减少了IECs中的主要基因相容性复合物II类 (MHCII) 和Ciita表达.
- 在MHCII和Ciita loci观察到可变和改变色素可访问性的减少.
- 补充剂恢复了MHCII转录水平,改善了炎症.
结论:
- Zip14介导的输送对于MHCII表达和免疫功能至关重要.
- 缺乏影响免疫基因的染色质调节.
- 准Zip14和水平可以预防肠道炎症疾病.
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