N6 - 甲基氨酸RNA甲基化调节彩虹鱼肝脏中微塑料诱导的细胞衰老
Fang Ma1, Zhe Liu2, Jinqiang Quan2
1School of Bioengineering and Technology, Tianshui Normal University, Gansu Province, PR China; Key Laboratory of Resource Utilization of Agricultural Solid Waste in Gansu Province, Tianshui Normal University, Tianshui, Gansu Province, PR China.
The Science of the total environment
|January 10, 2025
概括
微塑料积聚在彩虹鱼的肝脏中,触发抗氧化防御,并导致细胞损伤. 这种塑料污染通过改变基因表达来影响鱼类的健康,导致细胞循环停止和衰老.
科学领域:
- 环境毒理学环境毒理学
- 水生生态学 水生生态学
- 分子生物学分子生物学
背景情况:
- 微塑料是水生生态系统中广泛存在的环境污染物.
- 鱼类容易受到微塑料的摄入和随后的毒理影响.
研究的目的:
- 为了研究彩虹鱼肝脏中的微塑料积累.
- 评估微塑料对鱼类氧化应激,肝脏反应和基因表达的影响.
主要方法:
- 彩虹鱼 (Oncorhynchus mykiss) 暴露于微塑料 (0和500微克/升) 在14天内.
- 对肝脏微塑料积累,抗氧化酶活性和组织学变化的分析.
- 转录组分析侧重于N6-甲基氨酸 (m6A) 读取器和相关的基因表达.
主要成果:
- 在肝脏中显著的微塑料积累.
- 增强抗氧化酶活性和观察到的组织病变.
- 改变m6A读者表达 (IGF2BP1,YTHDF2),减少mRNA稳定性,减少细胞周期调节者的表达 (例如C-myc).
结论:
- 微塑料在彩虹鱼中诱导氧化应激和肝损伤.
- 暴露于微塑料会破坏与细胞周期控制和增殖有关的基因表达.
- 这项研究阐明了微塑料的毒理机制,强调了塑料污染对淡水生物的风险.
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