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核伪包容与BRAFV600E突变有关:对乳头甲状腺癌核特征的分析
Agnes Stephanie Harahap1, Dina Khoirunnisa2, Salinah3
1Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia/Dr. Cipto Mangunkusumo Hospital, Jakarta 14320, Indonesia; Human Cancer Research Center-Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta 14320, Indonesia.
Annals of diagnostic pathology
|January 10, 2025
概括
乳头甲状腺癌 (PTC) 亚型,BRAFV600E和RAS突变,表现出明显的核特征. 核伪含有是BRAFV600E的一个关键预测因素,有助于精确的亚型定制为量身定制的患者管理.
科学领域:
- 在瘤学瘤学.
- 病理学 病理学 病理学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 乳头甲状腺癌 (PTC) 是最常见的甲状腺癌,其中包括BRAF类 (BL) 和RAS类 (RL) 的亚型.
- 核变化是PTC的关键诊断标准,特别明显在BL亚型中.
- 了解像BRAFV600E和RAS突变这样的分子驱动因素是分类PTC的关键.
研究的目的:
- 为了研究和比较BRAFV600E突变和RAS突变PTC之间的组织核特征.
- 评估特定核特征在区分这些PTC亚型的诊断实用性.
- 为了确定核特征预测BRAFV600E突变状态.
主要方法:
- 对40个BRAFV600E突变和40个RAS突变的PTC样本进行了回顾性分析.
- 使用世卫组织 (3分) 和亚洲甲状腺工作组 (8分) 评分系统对核特征的组织学评估.
- 两变量和多变量统计分析以确定关联和预测值.
主要成果:
- 与RAS突变的PTC相比,BRAFV600E突变的PTC表现出更明显的核特征,包括膜不规则,延长,槽,形状和伪包含.
- 较高的核分数与BRAFV600E突变有显著的关联.
- 多变量分析确定核伪包含是BRAFV600E突变 (OR=10.97) 的重要预测因子,具有高特异性 (92.5%) 和准确性 (73.8%).
结论:
- 在BRAFV600E和RAS突变PTC之间存在明显的核形态差异.
- BRAFV600E突变的PTC呈现出花的核特征,而RAS突变的PTC显示出更微妙的变化.
- 核伪包含是识别BRAFV600E突变PTC的有价值的细胞学标记,支持针对性治疗的精确亚型.
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