胚胎的生物化学标受到兰巴-甲和伊米达克洛普里德的次致命度的影响
Nagat M Aly1, Anter K Mahmoud1, Eman M Mosallam1
1Mammalian and Aquatic Toxicology Department, Central Agricultural Pesticide Lab, Agricultural Research Center, Alexandria, Egypt.
Research in veterinary science
|January 10, 2025
概括
杀虫剂Lambda-cyhalothrin (LCT) 和imidacloprid (IMD) 的低于致命度对正在发育的小胚胎造成了重大伤害. 这些广泛使用的农业农药破坏了关键的酶活动和蛋白质水平,表明即使在低剂量也可能对发育有毒.
科学领域:
- 环境毒理学环境毒理学
- 发展生物学 发展生物学
- 农业科学 农业科学
背景情况:
- 兰巴-甲 (LCT) 和伊米达克洛普里德 (IMD) 是埃及农业中普遍存在的杀虫剂.
- 小胚胎 (Gallus Gallus) 作为评估毒理影响的敏感模型.
- 低于致命的农药暴露可以对发展中生物产生显著的,但往往被低估的影响.
研究的目的:
- 调查LCT和IMD在小胚胎发育中的亚致死度的毒理影响.
- 评估这些杀虫剂对关键生物化学标记物的影响,包括酶活性和蛋白质概况.
主要方法:
- 在化第四天,蛋被暴露在LCT和IMD的水溶液中,其度为两次次.
- 测量了大脑组织中的乙胆酶 (AChE) 活性.
- 分析了血清酶活动 (AST,ALT,ALP),酸酸酶 (ACP),谷氨S转移酶 (GST),肌素和总蛋白质含量. 使用SDS-PAGE评估了蛋白质配置文件.
主要成果:
- 在LCT和IMD暴露时观察到大脑ACHE活动的显著减少,剂量取决于剂量.
- 杀虫剂改变了血清中的AST和ALT活动,AST/ALT比率没有显著变化. 随着IMD,GST和ACP活动的增加.
- 在所有治疗组中,血清酶活性显著下降. 肌素和总蛋白质含量增加,以及SDS-PAGE检测到的蛋白质配置的变化.
结论:
- 即使低于致命的LCT和IMD度也对小胚胎发育构成重大风险.
- 观察到的生物化学变化表明可能会导致严重损伤和发育障碍.
- 这项研究强调,需要对农药使用进行仔细的监管和评估,以保护脆弱的胚胎阶段.
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