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HGF-HGFR-STAT信号轴调节Crassostrea gigas中的血细胞增殖

Rui Liu¹, Yuqing Zeng¹, Xue Qiao¹

¹Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.

Developmental and comparative immunology

|January 10, 2025

概括

中的类似肝细胞生长因子 (CgHGF-like) 和其受体 (CgHGFR) 调节血细胞的增殖. 这种相互作用激活了CgSTAT在对Vibrio splendidus的免疫反应期间,这对健康至关重要.

科学领域:

海洋生物学海洋生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

肝细胞生长因子 (HGF) 和它的受体 (HGFR) 对各种生物体的细胞生长和分裂至关重要.
,像Crassostrea gigas一样,拥有免疫细胞 (血细胞),对抗病原体至关重要.
了解免疫的分子机制对于水产养殖和生态系统健康至关重要.

研究的目的:

在Crassostrea gigas.中识别和表征HGF类 (CgHGF类) 和HGFR (CgHGFR) 同类物.
研究血细胞中CgHGF-like和CgHGFR的表达模式和相互作用,特别是在免疫刺激期间.
阐明CgHGF类/CgHGFR通路在调节中的血细胞增殖和免疫反应中的作用.

主要方法:

在Crassostrea gigas.中识别和对CgHGF类和CgHGFR基因的域分析.
定量实时PCR (qRT-PCR) 用于分析各种组织中的mRNA表达和在刺激Vibrio splendidus后.
免疫光测试以确定血液细胞内的蛋白质定位.
生物层干涉测量以确认复合CgHGF样蛋白和CgHGFR蛋白之间的体外相互作用.
通过RNA干扰 (RNAi) 来降低CgHGF样和CgHGFR表达,然后进行EDU整合试验和针对免疫相关基因的qRT-PCR (CgGATA,CgSCL,CgRUNX,CgSTAT).

主要成果:

在中发现了类似CgHGF和CgHGFR的同类物,在血细胞中mRNA表达最高.
这两种基因的表达在刺激Vibrio splendidus时在血细胞中显著增加.
在体外测试证实了CgHGF样和CgHGFR之间的相互作用.
通过RNAi介导的CgHGF类或CgHGFR降低了血细胞增殖 (EdU阳性细胞) 和细菌挑战后降低了关键免疫基因.
在细菌刺激后,CgHGF-like/CgHGFR-knockdown中抑制了关键信号分子CgSTAT的核转位.

结论:

这项研究确定了肝细胞生长因子途径在免疫力中的新作用.
类似CgHGF的蛋白与CgHGFR相互作用,通过在免疫反应期间激活CgSTAT信号来促进血细胞增殖.
这条通路对于对细菌感染的防御至关重要,突出显示了它在天生的免疫力中的重要性.

关键词:

细胞的增殖细胞的增殖.这种植物是Crassostrea gigas.在HGF的基础上.在HGFR的基础上,HGFR 血球细胞是血液细胞的组成部分.

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