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在太平洋海 (Haliotis discus hannai Ino) 和巨型海 (H. gigantea Gmelin) 中对VERL多态的内部变异和功能研究

Hanjiao Luo¹, Yi Wang¹, Jie Huang¹

¹State Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.

International journal of biological macromolecules

|January 10, 2025

概括

像VERL这样的配体识别蛋白 (GRPs) 在鱼中显示出高度的多样性,影响受精成功. 这项研究揭示了非常.

科学领域:

生殖生物学生殖生物学
海洋无脊椎动物遗传学海洋无脊椎动物遗传学
分子进化的分子进化.

背景情况:

配体识别蛋白 (GRPs) 调解物种特异性受精.
在GRPs中的多态性有助于配体亲和力和繁殖成功的变化.
了解GRPs对于海洋无脊椎动物繁殖计划至关重要.

研究的目的:

确定VERL的全长序列,VERL是卵膜对精子素的受体,在太平洋海 (Haliotis discus hannai Ino) 和巨型海 (Haliotis gigantea Gmelin) 中.
研究VERL的等位基多样性和内部特异变异,特别是在域重复中.
探索VERL多态性对受精潜力和分类交配的功能影响.

主要方法:

在两个海物种中对VERL的全长cDNA测序.
从重新测序数据中分析核酸多态度和哈普洛型分辨率.
对VERL域的内特异变异分析重复1-2.
基于VERL基因型的各种交配模式的调查.

主要成果:

确定了太平洋海 (11,373 bp,3790氨基酸,21次重复) 和巨型海 (9405 bp,3134氨基酸,17次重复) 的全长VERLcDNA序列.
在两种物种的VERL域重复1-2中观察到高等位基因多样性.
太平洋海的VERL基因组聚集在两个基因组中,没有显著的种群分歧,而巨型海基因组显示出截然不同的分支,多达12种氨基酸替代.
基于VERL基因型的选择性交配的第一个证据是在巨型海中发现的.

结论:

在VERL中的氨基酸多态性显著影响了 abalone 的受精潜力.
在太平洋和巨型海中发现了极大的等位基因多样性和独特的进化模式.
这些发现支持VERL在配体识别中的作用,并为选择性繁殖提供了基础,以提高鱼的繁殖效率.

关键词:

在受精过程中,受精配体识别蛋白质是一种配体识别蛋白质.哈利奥蒂斯讨论汉奈伊诺伊诺哈利奥蒂斯巨型Gmelin.这是 VERL VERL 的意思.

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