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相关概念视频

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Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
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相关实验视频

Updated: Jun 3, 2025

Lateral Root Inducible System in Arabidopsis and Maize
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miR166e/ZmATHB14模块有助于提高玉米根的耐旱性.

Xiaotong Wei1, Chunlai Wang1, Yimeng Wang1

  • 1College of Agronomy, Jilin Agricultural University, Changchun 130118, China; Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

International journal of biological macromolecules
|January 10, 2025
PubMed
概括

玉米miR166e对于耐旱性至关重要. 击败miR166e通过调节ZmATHB14转录因子来增强抗旱能力,改善血管发育和ROS恒温.

关键词:
干旱 干旱 干旱 干旱玉米 玉米 玉米 是 一种这就是ROSOS ROS.血管发育 血管发育miR166 是一个166个字母.

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Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
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科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 微RNAs (miRNAs) 是植物对环境压力的反应的关键调节者.
  • 玉米miR166是一种参与非生物应激反应的保存miRNA.
  • 在玉米根干旱应激反应中,miR166e的具体作用尚不清楚.

研究的目的:

  • 调查miR166e在玉米根干旱应激耐受性中的功能.
  • 为了确定参与干旱反应的miR166e的目标基因.
  • 阐明 miR166e-目标基因模块在干旱耐受性中的调节机制.

主要方法:

  • 定量实时PCR测量基因表达.
  • 对miR166e.进行基因操纵 (敲除和过度表达).
  • 5'-RACE和双露西法酶测定用于识别miRNA目标.
  • 在干旱压力下分析ZmATHB14表达和功能.
  • 液体染色学-质谱学 (HPLC-MS) 用于代谢分析.

主要成果:

  • 干旱压力减少了玉米根中的miR166e前体的表达.
  • miR166e敲击增强了干旱耐受性,而过度表达降低了它.
  • 确定HD-Zip III转录因子ZmATHB14是miR166e的直接标.
  • ZmATHB14积极调节干旱耐受性,其表达与干旱下的miR166e相反相关.
  • 在miR166e-ZmATHB14模块影响血管发育,ROS平衡,信号通路,和荷尔蒙水平.

结论:

  • 在调节玉米根干旱耐受性方面,miR166e-ZmATHB14模块起着至关重要的作用.
  • miR166e作为负调节剂,而它的标ZmATHB14则作为干旱耐受性的正调节剂.
  • 这个调节模块在干旱条件下影响关键的生理过程,包括血管发育和氧化应激管理.