在热应力下蛋白蛋白:热聚合方向和凝特性下降
Jingnan Zang1, Mingmin Qing1, Yujie Chi1
1College of Food Science, Northeast Agricultural University, Harbin 150030, China.
International journal of biological macromolecules
|January 10, 2025
概括
60°C以上的高热应力会对蛋白凝 (EWG) 特性产生负面影响. 温度升高会降低质地,保持水分的能力,以及由于蛋白质聚合而导致的稳定性.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 材料科学 材料科学 材料科学
背景情况:
- 蛋白蛋白是重要的食品成分.
- 了解热引起的变化对于加工至关重要.
- 蛋白质聚合会影响功能性质.
研究的目的:
- 为了研究热应激对蛋白凝 (EWG) 特性的影响.
- 为了确定财产退化的临界温度值.
- 阐明改变了EWG属性的结构基础.
主要方法:
- 控制的热应力应用 (56°C至76°C).
- 对凝质感,水能力和冷-解稳定性的分析.
- 硬度的风湿学分析.
- 对结构变化的光谱方法 (紫外线,光).
- 蛋白质聚合的微结构分析.
主要成果:
- 高于60°C的温度降低了EWG质地,保持水分的能力和稳定性.
- 在76°C时,自由水增加,刚度降低约48.6%,α-螺旋体含量降低约20.1%.
- 热应激诱导了蛋白质聚合,无序的集群和多孔的凝网络,导致水释放.
结论:
- 升高的热应力促进了EWG中的蛋白质聚合和结构障碍.
- 这种聚合导致一个多孔的网络,解释了减少的纹理和保持水分.
- 结果为优化蛋白产品加工和减轻聚合提供了洞察力.
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