在胃消化过程中微生物转胺酶修饰的鱼类凝的渐进结构变化
Ting Fang1, Zong-Cai Tu2, Hui Wang3
1National R&D Center for Freshwater Fish Processing, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi, 330022, China.
International journal of biological macromolecules
|January 10, 2025
概括
鱼类凝在模拟胃消化过程中经历结构变化. 微生物转质氨酶 (MTGase) 交联影响这些变化,影响原蛋白补充剂的开发.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 材料科学 材料科学 材料科学
背景情况:
- 鱼类凝是一种有价值的原蛋白来源,在补充剂中具有潜在的应用.
- 了解其在消化过程中的行为对于产品开发至关重要.
- 微生物转质氨酶 (MTGase) 交联可以改变凝的特性.
研究的目的:
- 在模拟胃消化过程中研究鱼类凝在热可逆 (TR) 和不可逆 (TI) 状态下的动态结构变化.
- 描述由MTGas交叉链接影响的消化模式.
- 为开发高质量的原蛋白补充剂提供见解.
主要方法:
- 在体外胃消化模拟.
- 分析自由氨基含量和SDS-PAGE用于水解.
- 表面水性和内源光测量.
- 微观结构观测和消化曲线分析.
主要成果:
- 素将TR和TI两种鱼类凝都化成更小的碎片.
- 疏水组嵌入TR样本,并在消化过程中暴露在TI样本中.
- 观察到明显的消化模式:TR样本稳定,而TI样本显示S形曲线.
- 一个图形模型说明了动态的结构变化.
结论:
- 在胃消化过程中,MTGas交叉连接显著改变了鱼类凝的结构动态.
- 观察到的疏水性和消化模式的变化对营养素的释放和生物可用性有影响.
- 这项研究支持开发具有可预测的消化性能的定制原蛋白补充剂.
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