相关实验视频

Updated: Jun 3, 2025

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

Published on: September 7, 2010

来自Neocaridina denticulata sinensis的一种新型L型乳清素 (NdLTL1) 的鉴定和功能分析

Yuewen Jia¹, Yijie Liu¹, Wenli Zhang¹

¹School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding, 071002, China.

Fish & shellfish immunology

|January 10, 2025

概括

这项研究揭示了NdLTL1,一种的乳素,与细菌碳水化合物结合,并促进免疫防御. 它结合了细菌,并显示了在免疫力和生长中的潜在作用.

科学领域:

海洋生物学海洋生物学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

的免疫力对水产养殖和生态系统健康至关重要.
莱克在天生的免疫力中发挥着至关重要的作用,通过识别病原体上的碳水化合物.
了解像NdLTL1这样的学蛋白,可以了解宿主-病原体相互作用.

研究的目的:

为了描述Neocaridina denticulata sinensis中的L型莱克NdLTL1.
为了研究其碳水化合物结合特异性和细菌凝结活性.
探索其在的免疫防御和细菌相互作用中的潜在作用.

主要方法:

莱克序列的生物信息学分析.
分子克隆,蛋白质表达和净化NDLTL1.
3D结构建模和蛋白质碳水化合物相互作用研究 (ELISA).
细菌结合测定和凝结试验.
对细菌挑战的反应中的基因表达分析 (qPCR).

主要成果:

NdLTL1有一个信号,一个跨膜域和一个Lectin_leg-like域.
它结合N-乙葡萄糖胺,丁糖甘,脂多糖 (LPS) 和曼诺糖.
再组合的NdLTL1以依赖的方式结合各种细菌菌株.
在暴露于Vibrio parahaemolyticus后,ndLTL1的表达在上升.
在体外,NdLTL1可能支持细菌生长,但也可能触发免疫反应.

结论:

NdLTL1是一种功能性L型单蛋白,参与识别细菌成分.
它表现出广泛的碳水化合物结合能力和细菌凝结活性.
NdLTL1可能在的天生的免疫力和宿主微生物相互作用中发挥着多方面的作用.

关键词:

细菌结合的结合方式糖分子的结合糖分子的结合这是天生的免疫力.这是一种L型乳清素.尼奥卡里迪纳 (Neocaridina) 是一种形形虫 (denticulata sinensis).

更多相关视频

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

Published on: January 6, 2017

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

相关概念视频

Selectins

Selectins

Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...