利用Ctb1的自然等位基和单基类型来提高大米的寒冷适应性
Lingling Li1, Gongye Cheng1, Wenyu Li1
1College of Agriculture, Hunan Agricultural University, Changsha, Hunan 410128, China.
Gene
|January 10, 2025
概括
米中的特定遗传变异在启动阶段提高了耐寒性. 这一发现涉及Ctb1基因,增强了在寒冷压力条件下水的弹性和产量.
科学领域:
- 植物科学 植物科学
- 遗传学 是一个遗传学.
- 农业科学 农业科学
背景情况:
- 在大米开采期间的寒冷压力显著降低了作物产量,特别是在较冷的气候下.
- 在启动阶段,CTb1基因在赋予寒冷耐受性方面发挥着至关重要的作用.
研究的目的:
- 为了研究大米中Ctb1-介导的寒冷耐受性的遗传基础.
- 为了确定Ctb1促进体中增强寒冷耐受性的特定遗传变异.
主要方法:
- 在202个米加入中重新测序Ctb1促销器.
- 单核酸多态 (SNPs) 和插入/删除 (InDels) 的分析.
- 开发和评估具有有益的内分线的近同位素线 (NIL-Ctb1HapI) 的开发和评估.
主要成果:
- 在Ctb1促销器中确定了6个INDEL和4个SNP.
- -1,302bp的InDel显着与Ctb1表达和耐寒性 (Haplotype I) 的增加有关.
- 在NIL-Ctb1HapI系中,Ctb1表达增加了5.9倍,增强了幼苗的存活率,提高了花粉的生育率,种植率提高了64.2%,在寒冷压力下增加了12g/植物的产量.
结论:
- 在Ctb1促进体中的1,302 bp InDel对于米的启动阶段寒冷耐受性至关重要.
- NIL-Ctb1 HapI 代表了一种有价值的遗传资源,用于培育耐寒米品种.
更多相关视频
06:10Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
7.3K
07:43Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
12.3K
相关概念视频
01:59Plant Breeding and Biotechnology
18.8K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
18.8K
02:45Responses to Heat and Cold Stress
13.3K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.3K
02:27Trihybrid Crosses
23.1K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
23.1K
02:50Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K
01:20Monohybrid Crosses
228.9K
Overview
228.9K