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通过在Methylorubrum populi中引入基酸盐合成酶突变来改善植物生长和水果质量

Cong Zhang^1,2,3, Lu Yao^1,4, Ming-Ming Zhang¹

¹School of Life Sciences, Qingdao Agricultural University, No.700 Changcheng Road, Chengyang District, Qingdao 266000, People's Republic of China.

Journal of applied microbiology

|January 10, 2025

概括

在Methylorubrum populi中引入基酸盐合成酶 (PRS) 突变,增强了植物界殖民和植物生长. 这种工程菌株显示出作为农业注射剂的承诺,用于提高作物产量和质量.

科学领域:

微生物生态学微生物生态学
植物与微生物的相互作用
农业生物技术农业生物技术

背景情况:

聚菌株具有促进植物生长的特征,包括酸生产, siderophore 合成,1-aminocyclopropane-1-carboxylate deaminase 活性和固定.
基因组分析证实了HS04菌株中对植物生长促进 (accD, aldB, ltaE) 和固定 (nifA, bchlLNB, bchXYZ) 关键基因的存在.

研究的目的:

为了研究基酸盐合成酶 (PRS) 突变对甲基菌的植物圈殖民的影响.
为了提高植物生长和质量,使用一种促进植物生长的转基因Methylorubrum菌株.

主要方法:

从花生叶子中分离和鉴定Methylorubrum populi株HS04的特征.
在HS04菌株中引入PRS突变 (D38N),形成HS04PTR.菌株.
评估HS04PTR在受控和温室条件下对黄瓜幼苗的植物界殖民和促进植物生长的影响.

主要成果:

在叶子上应用HS04显著增加了黄瓜苗的新鲜重量43.0%.
在PRS突变株HS04PTR中,低度甲醇和C2-C5碳来源的利用率提高.
HS04PTR证明了植物界殖民的改善,并进一步促进了黄瓜的生长,导致维生素C的增加,减少糖和总糖.

结论:

具有PRS突变的methylorubrum菌株是农业应用的有效免疫剂.
改造的Methylorubrum菌株为提高作物产量和质量提供了一个有希望的策略.

关键词:

梅西洛鲁布鲁姆的人民黄瓜黄瓜的使用方法基酸酸盐合成酶是一种基基酸.植物界的殖民化殖民化.质量改善质量改善

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