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Updated: Jun 3, 2025

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在数千个单细胞中对miRNAs和mRNAs的综合分析

Jia Li1, Jing Tian1, Tao Cai2,3

  • 1National Institute of Biological Sciences, Beijing, China.

Scientific reports
|January 10, 2025
PubMed
概括

这项研究引入了PSCSR-seq V2,一种用于同时分析单细胞中的微RNA (miRNA) 和信使RNA (mRNA) 的新方法. 这种技术使得大规模的联合表达分析成为可能,揭示了对细胞调节和衰老的洞察力.

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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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