使用九种不同的玉米酒对Ogataea polymorpha的代谢研究
Sekar Mayang W Wahjudi1, Dominik Engel1, Jochen Büchs2
1Aachener Verfahrenstechnik - Biochemical Engineering, RWTH Aachen University, Forckenbeckstr. 51, 52074, Aachen, Germany.
BMC biotechnology
|January 10, 2025
概括
玉米液 (CSL) 为Ogataea polymorpha发酵提供了一个具有成本效益的营养来源. 通过使用呼吸活动监测系统 (RAMOS) 和微板 (μTOM) 来检测CSL的批量变化,以优化培养.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物工艺工程 生物工艺工程
背景情况:
- 对于发酵中具有成本效益的营养来源的需求不断增加.
- 玉米液 (CSL) 是一种来自废物流的有希望的替代营养来源.
- 与CSL相关的挑战:批量变化和组成不一致.
研究的目的:
- 为了发展一个
- 使用指纹进行指纹采集.
- 系统用于检测CSL的批量变化.
- 使用CSL优化Ogataea多态植物种植.
主要方法:
- 使用了为震动瓶设计的呼吸活动监测系统 (RAMOS).
- 采用96孔微型板 (μTOM) 进行高通量选.
- 进行有针对性的营养补充,以确定局限性.
主要成果:
- 鉴定了氨作为CSL中的限制基质,通过硫酸补充物克服了这种局限性.
- 在氨限制下观察到O. polymorpha的形态变化和改变细胞大小分布.
- 确定,由于微量营养素 (铜,铁,) 的降水,增加CSL储存时间会对种植产生负面影响.
结论:
- CSL是O. polymorpha的一种多功能营养来源.
- "指纹" CSL 批次可以进行早期选和选择最佳批次.
- 通过选择优质的CSL批量或针对性地补充劣质批量,可以减轻发酵不一致性.
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