响应表面方法和重复批次发酵策略用于增强使用皮废弃物从海洋油性Candida parapsilosis Y19增加脂质的生产
AbdAllah M Matouk1, Gadallah M Abu-Elreesh2, Mohamed Ali Abdel-Rahman1
1Botany and Microbiology Department , Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Microbial cell factories
|January 10, 2025
概括
海洋酵母Candida parapsilosis Y19有效地从皮中产生脂质. 优化和重复批次发酵显著提高了脂质产量和含量,产生了高含量的不和脂肪酸,如油酸.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
背景情况:
- 油性酵母是生产脂质的关键微生物资源.
- 海洋酵母为工业应用提供了独特的代谢能力.
- 有效的脂质积累需要优化种植条件.
研究的目的:
- 隔离和识别具有优越脂质积累能力的海洋油性酵母.
- 使用统计优化和发酵策略,提高选定酵母菌株的脂质产量.
- 描述产生的脂质的脂肪酸特征.
主要方法:
- 使用尼罗河红色染料分离和选海洋油性酵母.
- 使用Taguchi设计的响应表面方法 (RSM) 优化种植参数.
- 重复批次发酵用于增强脂质生产.
- 用于脂肪酸分析的气相色谱分析.
主要成果:
- 鉴定出Candida parapsilosis Y19是最强大的产生脂质的海洋酵母.
- 优化条件使总脂质增加到2.46g/l,脂质含量增加到30.7%.
- 重复批次发酵进一步提升了总脂质到4.78g/l (4.19倍增加) 和含量到39.1% (1.7倍增加).
- 产生的脂质富含不和脂肪酸 (74.8%),主要成分为油酸 (45.0%) 和酸 (26.0%).
结论:
- 坎迪达 (Candida parapsilosis) Y19是微生物脂质生产的一个有希望的候选者.
- 通过RSM进行统计优化和重复批次发酵是最大限度地提高脂质产量的有效策略.
- 酵母脂质具有有利的脂肪酸特征,适合各种应用.
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