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基于计算机辅助精液分析和流细胞计的野猪精液特征的全基因组关联分析

Xiyan Yang¹, Jingkun Nie¹, Yaxuan Zhang¹

¹School of Life Science and Engineering, Foshan University, Foshan 528000, China.

Animals : an open access journal from MDPI

|January 11, 2025

概括

这项研究确定了与野猪精液质量特征相关的遗传标记物和候选基因,包括运动性和密度. 这些发现可以通过有针对性的选择来提高养猪效率和精子质量.

科学领域:

动物遗传学动物遗传学
生殖生物学生殖生物学
量化遗传学量化遗传学

背景情况:

精液质量对于养猪人工授精至关重要.
评估各个野猪的生育能力会影响养殖计划的效率和经济成果.

研究的目的:

进行基因组广泛关联研究 (GWAS) 关于杜罗克野猪的关键精液特征.
识别影响精子质量和持久性的遗传变异和候选基因.

主要方法:

通过流细胞计 (FCM) 和计算机辅助精子分析 (CASA) 评估精液特征 (运动性,密度,异常性,线粒体膜潜力,宏体完整性,反应性氧物种).
通过使用GeneSeek GGP Porcine 50 K SNP BeadChip进行了1183只Duroc野猪的基因定型.
使用通用线性模型 (GLM),混合线性模型 (MLM) 和农场CPU方法执行GWAS.
使用单个和多个特征PBLUP模型估计的遗传性.

主要成果:

对大多数精液特征 (MOT,DEN,MMP,AIR,ROS) 确定了低遗传性,对异常 (ABN) 具有中等遗传性.
检测到9个候选基因 (例如GPX5,AWN,PSP-II),可能与精液特征相关.
这些基因与猪精子中的抗氧化和线粒体功能有关.

结论:

这项研究阐明了Duroc猪精液特征的遗传结构.
已识别的SNP和候选基因为提高养猪经济成果提供了潜力.
这些发现支持有针对性的育种策略,以提高猪精子质量.

关键词:

在家里,房子.在GWAS中,GWAS就是GWAS.杜罗克野猪是野猪的动物.流动细胞计量是流动细胞计量的方法.精液的特征精液的特征

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