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维佩林及其对常见鱼 (Cyprinus carpio) 的SVCV复制的影响

Yan Meng¹, Xi Hu¹, Nan Jiang¹

¹Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.

Animals : an open access journal from MDPI

|January 11, 2025

概括

常见的鱼毒毒素 (cc-viperin),是一种干扰素刺激的基因,对鱼病毒 (SVCV) 的春季病毒病有抗病毒特性. 过度表达cc-viperin可以抑制鱼类细胞中的SVCV复制.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
水生动物卫生水生动物卫生

背景情况:

维佩林是一种干扰素刺激基因 (ISG),对脊椎动物的先天抗病毒免疫是至关重要的.
鱼病毒 (SVCV) 的春季病毒症对水产养殖构成重大威胁,需要对抗病毒机制进行研究.

研究的目的:

为了表征常见的鱼毒蛇 (cc-viperin) 基因.
为了研究cc-viperin对EPC细胞中SVCV复制的抗病毒活性.

主要方法:

全长cDNA测序和cc-viperin的测序分析.
遗传学分析和亚细胞局部化研究.
基因表达分析和功能测定涉及SVCV感染和cc-viperin操纵.

主要成果:

cc-viperin基因编码了一个348氨基酸蛋白质,具有保存域 (氨酸拉链,SAM域).
cc-viperin与其他物种的viperin具有很高的序列相同性,并且在细胞质中定位.
在SVCV感染期间,cc-viperin的表达上调,并且在过度表达时显著抑制病毒复制,而减少表达则会增强病毒复制.

结论:

cc-viperin与脊椎动物的viperin orthologs共享保存的特征.
cc-viperin在普通鱼 (EPC细胞) 中显示出对SVCV的显著抗病毒作用,突出显示其在鱼类抗病毒免疫力中的作用.

关键词:

抗病毒活动抗病毒活动它们的特性,特点.常见鱼 (Cyprinus carpio) 是一种常见的鱼类.维珀林是一种毒蛇.

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