相关实验视频

Updated: Jun 3, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

OsBBX2通过抑制Hd3a在米中长日条件下的表达来延迟开花

Yusi Yang¹, Jiaming Wei^2,3, Xiaojie Tian²

¹College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China.

Plants (Basel, Switzerland)

|January 11, 2025

概括

米BBX2 (OsBBX2) 蛋白质在根中表达很高,通过与Hd1相互作用并抑制Hd3a转录,延迟了开花. 这一发现对于理解水生长和发展规则至关重要.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
遗传学和基因组学在

背景情况:

B-Box (BBX) 家族蛋白质是大米 (Oryza sativa) 生长和发育的重要调节者.
了解个别BBX蛋白的特定作用是破译大米复杂发育途径的关键.

研究的目的:

确定和描述一种新型的米BBX蛋白,Oryza sativa BBX2 (OsBBX2),重点关注其在开花时间调节中的作用.
阐明OsBBX2影响大米开花时间的分子机制.

主要方法:

对OsBBX2的识别和表达分析,包括其昼间节律.
通过过度表达OsBBX2 (OsBBX2 OE) 和在大米中产生bbx2突变的功能分析.
分子机制研究涉及蛋白质-蛋白质相互作用测试 (OsBBX2与Hd1) 和基因表达分析 (Ehd1,Hd3a,RFT1).

主要成果:

OsBBX2表现出转录抑制活性,在米根中表达很高,白天转录在黎明时达到峰值.
在长日条件下,OsBBX2过度表达 (OsBBX2 OE) 显著延迟了标题日期,而bbx2突变体显示了野生类型的开花.
OsBBX2与Hd1相互作用,增强Hd3a转录的抑制,导致关键的开花基因 (Ehd1,Hd3a,RFT1) 的下调.

结论:

OsBBX2作为水开花时间的负调节剂.
OsBBX2蛋白质通过与Hd1形成复合物来延迟开花,该复合物与Hd1一起抑制花整合基因Hd3a的转录.
这项研究揭示了一种涉及OsBBX2和Hd1的新型分子途径,用于控制水光周期开花.

关键词:

BBX2 BBX2 BBX2 BBX2 BBX2 BBX2 BBX2 BBX2 BBX2 在 Hd1 中,Hd1 是 Hd1 .在Hd3a中,它是Hd3a.标题日期标题日期标题日期米米饭米饭米饭米饭.

更多相关视频

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

相关概念视频

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...