聚甲酸的消毒策略:方法序列,溶液度和口内温度对抗菌活性的影响
Ana Beatriz Sato Kamio1, Andressa da Silva Barboza1, Maria Eduarda Broering da Silva1
1Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s/n-Trindade, Florianópolis 88040-900, SC, Brazil.
Polymers
|January 11, 2025
概括
结合刷牙和0.25%的化 (NaOCl) 浸泡是对抗 Candida albicans 的假牙消毒最有效的方法. 消毒后的口内温度暴露促进了重新定居,降低了抗菌药物的有效性.
科学领域:
- 牙科材料科学 牙科材料科学
- 微生物学 微生物学
- 传染性疾病 传染性疾病
背景情况:
- 牙生物膜积累,特别是Candida albicans,对口腔健康构成重大风险.
- 有效的假牙消毒方案对于预防假牙口炎和全身感染至关重要.
研究的目的:
- 评估各种假牙消毒方案对Candida albicans的抗菌有效性.
- 评估化 (NaOCl) 度,干预序列和消毒后口腔内温度对消毒有效性的影响.
主要方法:
- 热聚合聚甲基酸 (PMMA) 标本经过不同的消毒协议:刷 (B),化 (NaOCl) 浸泡 (I) 在0.1%和0.25%,组合方法 (B+I,I+B),并包括消毒后的口内温度暴露 (T).
- 定量分析涉及对Candida albicans的殖民地形成单位 (CFU/mL) 计数.
- 定性分析使用扫描电子显微镜 (SEM) 来可视化残留生物膜.
主要成果:
- 结合机械 (刷毛) 和化学 (0.25% NaOCl) 消毒方案,在降低Candida albicans数量方面表现出最高的有效性.
- 当浸泡在刷牙之前时,0.1%的NaOCl的疗效降低了.
- 消毒后暴露于口内温度 (模拟睡眠条件) 导致Candida albicans重殖民,降低了所有测试协议的有效性.
结论:
- 使用0.25%的低酸盐 (NaOCl) 的组合方案是最有效的消毒假牙对Candida albicans.
- 机械和化学消毒的序列会影响较低NaOCl度 (0.1%) 的疗效.
- 口腔内温度暴露显著影响消毒后的微生物重新定居,强调需要全面的假牙卫生策略.
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