在羊群卵细胞复合体上DEHP和混合物的体外毒性
Antonella Mastrorocco1, Letizia Temerario1, Valeria Vurchio1
1Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy.
International journal of molecular sciences
|January 11, 2025
概括
一种混合的二二甲基甲酸 (DEHP) 和 (Cd) 显著损害羊卵细胞质量比单个化合物. 这种混合物影响细胞能量和氧化应激,影响线粒体健康.
科学领域:
- 生殖毒理学 生殖毒理学
- 环境健康 环境健康
- 细胞生物能量学 细胞生物能量学
背景情况:
- 已知二二甲基甲酸 (DEHP) 和 (Cd) 是已知的生殖毒素.
- 生物通常暴露在污染物的混合物中,而不是单一的化合物.
- 关于DEHP和Cd对女性生殖细胞的联合毒理作用的研究有限.
研究的目的:
- 为了研究DEHP/Cd混合物对绵羊积卵细胞复合体 (COC) 成熟的作用.
- 为了比较DEHP/Cd混合物与单个DEHP和Cd暴露的影响.
- 评估COC中的核和细胞质成熟,生物能和氧化参数.
主要方法:
- 从从屠宰场获得的卵巢中恢复了绵羊的COCs.
- 在体外成熟 (IVM) 过程中,COCs在体外暴露于0.5μM DEHP,0.1μM Cd或DEHP/Cd混合物24小时.
- 评估了卵细胞核染色素,线粒体分布,膜潜力,活性氧物种 (ROS) 水平和线粒体/ROS联合定位.
主要成果:
- 在任何测试条件下,卵细胞核成熟没有受到影响.
- 与单个化合物相比,DEHP/Cd混合物显著降低了健康线粒体分布的卵细胞百分比.
- 暴露于混合物和单一化合物减少了卵细胞线粒体膜潜力,细胞内ROS和线粒体/ROS共定位;然而,积细胞仅在混合物暴露时显示ROS增加.
结论:
- 在体外暴露于DEHP/Cd混合物对绵羊的COC质量产生负面影响比单个DEHP或Cd暴露更严重.
- 这种混合物破坏了COC的生物能量和氧化平衡,特别是影响了线粒体健康和累积细胞中的ROS水平.
- 这些发现凸显了在生殖毒理学中研究组合毒性作用的关键需要.
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